SPE及纳米金涂层毛细管电泳检测血浆中土霉素与多西环素

发布时间：2018-06-07 00:11

本文选题：毛细管电泳 + 土霉素　；参考：《分析测试学报》2015年09期

【摘要】：建立了混合型固相萃取及季铵化纤维素负载的纳米金涂层毛细管电泳法快速检测血浆中土霉素和多西环素的方法。采用季铵化纤维素负载的纳米金复合材料(QC-Au NPs)对毛细管内壁进行动态涂层,以抑制管壁对分析物的吸附,并对缓冲液p H值进行优化。结果表明,该涂层能改善峰形和分离度,提高分离效率,其中土霉素的柱效提高了17.9倍。在涂层毛细管中,土霉素和多西环素的吸附被抑制,并在4 min内出峰。在10~200μg/m L范围内,土霉素和多西环素的峰面积与浓度的线性关系良好,相关系数(r)分别为0.997 6和0.995 2。血浆中土霉素和多西环素的加标回收率为88%~107%,相对标准偏差(RSD)为1.4%~7.7%。该方法快速、简便,准确度和精密度高,适用于血浆中土霉素和多西环素的快速检测。
[Abstract]:A method for rapid determination of oxytetracycline and doxycycline in plasma by capillary electrophoresis with nano-gold coated with mixed solid phase extraction and quaternary ammonium cellulose was established. Quaternary cellulose supported gold nanocomposite (QC-Au NPsNPs) was used to dynamically coat the inner wall of capillary to inhibit the adsorption of the analyte and optimize the pH value of buffer solution. The results showed that the coating could improve the peak shape, separation degree and separation efficiency, and the column efficiency of oxytetracycline was increased by 17.9 times. In the coated capillary, the adsorption of oxytetracycline and doxycycline was inhibited and the peak was obtained within 4 min. The linear relationship between the peak area and the concentration of oxytetracycline and doxycycline was good in the range of 10 渭 g / mL with correlation coefficients of 0.997 6 and 0.995 2, respectively. The recoveries of oxytetracycline and doxycycline in plasma were 88 and 107, and the relative standard deviation (RSD) was 1.4 and 7.7. The method is rapid, simple, accurate and accurate. It is suitable for the rapid determination of oxytetracycline and doxycycline in plasma.
【作者单位】：深圳市福田区疾病预防与控制中心;广东药学院药科学院;
【基金】：深圳市科技研发基础研究项目(JCYJ20140414100411116) 福田区卫生公益性科研项目(FTWS201304,201458)
【分类号】：R96;O658.9

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