西酞普兰及其代谢产物的毛细管电泳手性拆分研究

发布时间：2018-06-07 07:52

  本文选题：西酞普兰 + 代谢产物　； 参考：《中国新药杂志》2017年22期

【摘要】：目的:建立西酞普兰及其代谢产物(去甲基西酞普兰及二去甲基西酞普兰)的高效毛细管电泳手性拆分方法。方法:以磺酸化-β-环糊精为手性选择试剂对西酞普兰及其代谢产物进行拆分,并考察了S-β-CD浓度、背景电解质的pH、有机溶剂的比例对手性分离的影响。结果:在pH为3.0,30 mmol·L-1磷酸二氢钠缓冲溶液,S-β-CD浓度为10 mg·m L-1,有机溶剂甲醇的浓度为30%,分离电压为20 k V,检测波长为205 nm,西酞普兰及其2个代谢产物均达到完全分离,其分离度分别为7.2,6.3,3.4。结论:该方法简便、快速,可用于西酞普兰、去甲基西酞普兰及二去甲基西酞普兰的手性分离。
[Abstract]:Objective: to establish a chiral separation method of citalopram and its metabolites (demethylcitalopram and dimethylcitalopram) by high performance capillary electrophoresis (HPCE). Methods: citalopram and its metabolites were separated with sulfonated 尾 -cyclodextrin as chiral selective reagent. The effects of concentration of S- 尾 -CD, pH of background electrolyte and proportion of organic solvent on chiral separation were investigated. Results: the concentration of S- 尾 -CD in sodium dihydrogen phosphate buffer solution was 10 mg mL ~ (-1) at pH 3.0 ~ 30 mmol ~ (-1), the concentration of organic solvent methanol was 30 鈩，

本文编号：1990423