栀子苷联合美满霉素拮抗PC12细胞凋亡的作用

发布时间：2018-06-07 14:25

本文选题：栀子苷 + 美满霉素　；参考：《神经解剖学杂志》2017年04期

【摘要】：目的:观察栀子苷和美满霉素联合应用对H_2O_2诱导PC12细胞凋亡的作用,并对其机制进行初步探讨。方法:处于对数生长期的PC12细胞随机分为对照组、模型组、栀子苷组、美满霉素组以及栀子苷/美满霉素联合应用组。用300μmol/L H_2O_2诱导6 h建立PC12细胞凋亡模型。MTT法测定细胞存活率,比色法测定乳酸脱氢酶(LDH)活性,倒置相差显微镜观察细胞形态,Hoechest 33258荧光核染色观察细胞凋亡状况,Annexin V-FITC/PI双染法流式细胞仪检测细胞凋亡率,Western Blot测定Caspase-3蛋白表达。结果:与模型组相比,栀子苷组和美满霉素组PC12细胞存活率提高,LDH释放量减少,细胞凋亡率降低,同时Caspase-3蛋白表达减少(P均0.05);与栀子苷组和美满霉素组相比,栀子苷/美满霉素联合应用组PC12细胞的存活率进一步提高,LDH释放量进一步减少,细胞凋亡率进一步降低,同时Caspase-3蛋白表达进一步减少(P0.05或P0.01)。以上指标相比,差异均有统计学意义。结论:栀子苷与美满霉素联合应用较单一应用能更有效拮抗PC12细胞凋亡,其机制可能与抑制Caspase-3蛋白表达有关。
[Abstract]:Aim: to observe the effect of geniposide combined with minocycline on apoptosis of PC12 cells induced by H_2O_2 and its mechanism. Methods: PC12 cells at logarithmic growth stage were randomly divided into control group, model group, geniposide group, minocycline group and combined application of geniposide and minocycline. The apoptosis model of PC12 cells induced by 300 渭 mol/L H_2O_2 for 6 h was established. The survival rate of PC12 cells was measured by MTT method, and the activity of lactate dehydrogenase (LDH) was measured by colorimetric method. Apoptosis was observed by inverted phase contrast microscope and fluorescence nuclear staining of Hoechest 33258. The apoptosis rate was detected by flow cytometry with Annexin V-FITC/PI double staining and the expression of Caspase-3 protein was detected by Western Blot. Results: compared with the model group, the survival rate of PC12 cells in geniposide group and minocycline group was decreased, the cell apoptosis rate was decreased, and the expression of Caspase-3 protein was decreased (P < 0.05), compared with those in geniposide group and minocycline group. In combination with geniposide / minocycline group, the survival rate of PC12 cells was further increased, the cell apoptosis rate was further decreased, and the expression of Caspase-3 protein was further decreased by P0.05 or P0.01. Compared with the above indexes, the differences were statistically significant. Conclusion: the combination of geniposide and minocycline can antagonize the apoptosis of PC12 cells more effectively than that of minocycline alone, and the mechanism may be related to the inhibition of Caspase-3 protein expression.
【作者单位】：漯河医学高等专科学校生理学教研室;漯河医学高等专科学校病理生理学教研室;
【基金】：河南省科技厅基础与前沿技术研究基金资助项目(152300410014) 河南省教育厅高等学校重点科研项目(16A180031) 漯河医学高等专科学校博士基金资助项目(2014DF001)
【分类号】：R96

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