咪达唑仑经鼻给药在最大电休克发作及戊四唑惊厥模型的抗惊厥作用

发布时间：2018-06-08 22:47

本文选题：咪达唑仑 + 地西泮　；参考：《军事医学》2015年05期

【摘要】：目的在大鼠最大电休克发作(MES)及戊四唑惊厥(MST)模型上,比较评价咪达唑仑与地西泮经鼻给药的抗惊厥作用。方法大鼠随机分为7组,分别为模型组、咪达唑仑鼻喷剂低、中、高剂量组和地西泮鼻喷剂低、中、高剂量组,每组10只。建立MES及MST模型,评价不同剂量咪达唑仑经鼻给药的抗惊厥作用,并与经典抗癫痫药物——地西泮比较,观察给药后72 h各组动物海马、皮质及杏仁核组织病理学变化。结果不同剂量咪达唑仑经鼻预防给药,均可剂量依赖性地对抗MES及MST;在等剂量给药条件下,其抗惊厥作用明显优于地西泮(P0.05或P0.01)。病理学结果显示,咪达唑仑与地西泮经鼻预防给药均可减轻癫痫所致海马、皮质及杏仁核神经元细胞的损伤,抑制小胶质细胞增多及炎性细胞浸润,细胞核固缩以及嗜神经现象减少。结论咪达唑仑经鼻给药,在经典癫痫模型上均具显著抗惊厥作用,并可显著抑制癫痫所致脑损伤。
[Abstract]:Objective to evaluate the anticonvulsant effects of midazolam and diazepam in rat models of maximal electroshock seizure (MES) and pentylenetetrazole (pentylenetetrazole). Methods Rats were randomly divided into 7 groups: model group, midazolam nasal spray group, middle dose group, high dose group and diazepam nasal spray group with 10 rats in each group. Mes and MST models were established to evaluate the anticonvulsant effects of midazolam at different doses by nasal administration. The histopathological changes of hippocampus, cortex and amygdala were observed 72 hours after administration of midazolam compared with the classical antiepileptic drug diazepam. Results different doses of midazolam could antagonize mes and MST in a dose-dependent manner, and the anticonvulsant effect of midazolam was significantly better than that of diazepam (P0.05 or P0.01). The pathological results showed that midazolam and diazepam could attenuate the injury of neurons in hippocampus, cortex and amygdala, inhibit the increase of microglia and the infiltration of inflammatory cells in hippocampus, cortex and amygdala induced by epilepsy. Nuclear pyknosis and neurophilic phenomenon decreased. Conclusion midazolam has a significant anticonvulsive effect on classical epileptic model and can significantly inhibit the brain injury induced by epilepsy.
【作者单位】：陕西中医学院药学院临床中药学教研室;军事医学科学院毒物药物研究所抗毒药物与毒理学国家重点实验室;
【基金】：全军医学科技“十二五”重大(AWS11C004)及重点项目(BWS12J042)
【分类号】：R965

【参考文献】