替米沙坦对兔髂动脉再狭窄模型新生内膜增殖的影响

发布时间：2018-06-09 21:59

  本文选题：再狭窄 + 血管平滑肌细胞　； 参考：《中华高血压杂志》2015年05期

【摘要】：目的观察兔髂动脉再狭窄模型病理改变,并加用替米沙坦进行干预,探讨其对再狭窄模型新生内膜增殖的影响。方法 30只新西兰大白兔随机分为对照、再狭窄和替米沙坦组,每组10只。采用高脂饲料喂养联合兔髂动脉二次球囊损伤建立再狭窄模型,其中替米沙坦组于第二次球囊损伤后第2天开始加用替米沙坦5mg/(kg·d)。实验结束时,动物处死前采血检测总胆固醇,并留取髂动脉标本,行苏木精伊红染色法染色及免疫组化检测,组织匀浆测定血管紧张素Ⅱ(AngⅡ)水平。结果与对照组比较,再狭窄和替米沙坦组总胆固醇[(20.44±0.68)、(20.39±0.60)比(0.58±0.19)mmol/L]、血管壁AngⅡ[(115.6±15.7)、(123.8±22.1)比(90.1±7.7)ng/L]、内膜厚度[(266.1±70.3)、(68.2±24.4)比(2.8±0.2)μm]和狭窄率[(89.3±6.9)%、(42.6±18.1)%比(23.0±3.5)%]增加(均P0.05)。而替米沙坦组的内膜厚度和狭窄率低于再狭窄组(均P0.01)。与对照和替米沙坦组比较,再狭窄组平滑肌细胞增殖率明显增加[(21.9±4.7)%比(7.3±4.9)%、(7.1±5.1)%,均P0.01]。α平滑肌肌动蛋白染色证实,再狭窄和替米沙坦组的新生内膜主要细胞成分为血管平滑肌细胞。结论替米沙坦对兔髂动脉再狭窄模型血管平滑肌细胞增殖及新生内膜增殖均有抑制作用。
[Abstract]:Objective to observe the pathological changes of rabbit iliac artery restenosis model and to investigate the effect of telmisartan on neointimal proliferation in restenosis model. Methods Thirty New Zealand rabbits were randomly divided into control group, restenosis group and telmisartan group with 10 rabbits in each group. The restenosis model of iliac artery was established by high fat diet feeding combined with secondary balloon injury of iliac artery in rabbits. Telmisartan 5mg/(kg dronium was added to telmisartan group on the second day after the second balloon injury. At the end of the experiment, the blood samples were collected to detect total cholesterol before the animals were killed, and the iliac artery specimens were collected. The blood samples were stained with hematoxylin eosin staining and immunohistochemistry, and the level of angiotensin 鈪，

本文编号：2000968