大鼠血浆中7-乙基-10-羟基喜树碱-10-棕榈酸酯及7-乙基-10-羟基喜树碱HPLC分析方法的建立

发布时间：2018-06-10 13:14

  本文选题：高效液相色谱法 + SN-PA　； 参考：《国际药学研究杂志》2015年05期

【摘要】：目的建立同时测定大鼠血浆中7-乙基-10-羟基喜树碱-10-棕榈酸酯(SN38-PA)及其活性代谢产物7-乙基-10-羟基喜树碱(SN38)含量的高效液相色谱法。方法以10-羟基喜树碱(10-CPT)作为内标,甲酸酸化血浆,甲醇沉淀蛋白。采用Agilent C18柱(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.2%甲酸水溶液为流动相进行梯度洗脱,流速1 ml/min,紫外检测波长372 nm,柱温:30℃。结果大鼠血浆中SN38-PA和SN38线性范围分别为0.25~62.5(r=0.9998)和0.05~12.5μg/ml(r=0.9997),最低定量限分别为0.18和0.04μg/ml;两组分平均绝对回收率分别为95.89%和97.03%;平均相对回收率分别为99.54%和99.84%。2组分日内、日间精密度的RSD%均3%。结论本分析方法快速、简便、准确度和灵敏度高,可用于SN38-PA及其活性代谢产物SN38的体内检测。
[Abstract]:Objective to establish a HPLC method for simultaneous determination of 7-ethyl-10-hydroxycamptothecin (7-ethyl-10-hydroxycamptothecin) and its active metabolite (7-ethyl-10-hydroxycamptothecin) in rat plasma. Methods 10-hydroxycamptothecin-10-CPT was used as internal standard. Plasma was acidified with formic acid and protein was precipitated by methanol. A Agilent C18 column (4.6 mm 脳 250 mm ~ 5 渭 m) was used for gradient elution with methanol-0.2% formic acid solution as mobile phase. The flow rate was 1 ml / min, UV detection wavelength was 372 nm, column temperature was 30 鈩，

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