不同粒径的大气细颗粒物对A549细胞的毒性研究

发布时间：2018-06-19 14:21

本文选题：环境科学技术基础学科 + 毒理学　；参考：《安全与环境学报》2017年05期

【摘要】：研究了不同粒径的大气细颗粒物对人肺癌上皮细胞A549的生物学效应,探讨了大气中细颗粒物粒径与细胞毒性的关系。采集杭州市大气中不同粒径的细颗粒物,制备成不同粒径的颗粒物总悬浮液,将A549细胞暴露于该制备液24 h后,测定细胞活力(MTT法)和培养液上清液中的LDH含量;并选择最佳浓度为后续暴露剂量,测定ROS生成量,评价不同粒径大气细颗粒物对细胞损伤效应。此外,还采用RTPCR测定凋亡基因P50、BAX、BCL-2表达情况。不同粒径的细颗粒物均显著抑制A549细胞活力,LDH显著增多,并存在剂量-效应关系;最佳染毒质量浓度为10μg/m L;ROS的生成量和各个凋亡基因的mRNA表达量均高于对照组。大气中不同粒径的细颗粒物均能对A549细胞产生毒性,并且随粒径增加,颗粒物对细胞的毒性呈降低趋势。
[Abstract]:The biological effects of atmospheric fine particles with different particle sizes on human lung cancer cell line A549 were studied and the relationship between particle size and cytotoxicity was investigated. The fine particles of different sizes in the atmosphere of Hangzhou were collected and the total suspensions of particles of different sizes were prepared. After 24 hours of exposure to the preparation solution, the LDH content in the supernatant of the culture medium and the cell viability MTT assay were measured. The optimal concentration was chosen as the subsequent exposure dose to determine the Ros production and to evaluate the damage effect of different particle sizes on the cells. In addition, the expression of apoptotic gene P50, Bax, BCL-2 was detected by RT PCR. The activity of LDH in A549 cells was significantly inhibited by fine particles of different sizes, and there was a dose-effect relationship, and the production of Ros and the mRNA expression of various apoptotic genes in A549 cells were significantly higher than those in the control group at the optimal concentration of 10 渭 g / m LLL- Ros. Fine particles of different sizes in the atmosphere could produce toxicity to A549 cells, and the toxicity of particles to A549 cells decreased with the increase of particle size.
【作者单位】：浙江工业大学环境学院;
【基金】：国家自然科学基金面上项目(21177114) 浙江省自然科学基金项目(LZ15B070001)
【分类号】：R994.6;X513

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