西咪替丁对低剂量电离辐射致大鼠氧化应激的保护作用

发布时间：2018-06-23 05:43

本文选题：西咪替丁 + 辐射　；参考：《解放军医学杂志》2017年02期

【摘要】：目的研究西咪替丁对低剂量照射所致大鼠氧化应激损伤的保护作用。方法研究分体内和体外实验。体内实验以SD大鼠作为研究对象,将60只大鼠按体重随机分为6组,即正常对照组、模型对照组、香菇多糖组(89mg/kg.d)及西咪替丁高中低三个剂量组(n=10)。每天于给药后辐照,辐照时间8h,连续12d,第13天处死动物。检测大鼠血清、肝脏、胸腺和脾脏中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)及丙二醛(MDA)。体外实验:采用超氧阴离子自由基体系、羟基自由基体系、过氧化氢自由基体系、烷基自由基引发的亚油酸氧化体系及二苯代苦味酰肼自由基(DPPH)自由基体系检测西咪替丁清除自由基的能力。结果体内实验结果显示,给予累积剂量为0.3Gy的低剂量电离辐射照射后,大鼠肝脏、胸腺中抗氧化酶SOD的活力显著下降,血清、肝脏、脾脏中GSH-Px活力明显下降,血清、肝脏、脾脏中MDA含量明显升高。而西咪替丁能不同程度地提升血清和各器官组织中抗氧化酶的活力,降低MDA含量。体外实验结果显示,在一定浓度范围内,西咪替丁对超氧阴离子自由基、羟基自由基、烷基自由基和DPPH 自由基均有不同程度的清除作用,对羟基自由基的清除能力最佳。结论西咪替丁能有效清除自由基,通过增强抗氧化酶活力和降低脂质过氧化产物含量,减轻低剂量累积照射诱导的大鼠氧化应激,显示了较好的辐射防护作用。
[Abstract]:Objective to study the protective effect of cimetidine on oxidative stress injury induced by low dose irradiation in rats. Methods the experiment was carried out in vivo and in vitro. Sprague-Dawley rats were randomly divided into 6 groups according to their body weight: normal control group, model control group, lentinan polysaccharide group (89mg/kg.d) and cimetidine medium and low dose group (nmm-10). The animals were killed on the 13th day after irradiation for 8 hours every day. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (cat) and malondialdehyde (MDA) were detected in serum, liver, thymus and spleen of rats. In vitro experiments: superoxide anion radical system, hydroxyl radical system, hydrogen peroxide free radical system, The ability of cimetidine to scavenge free radicals was detected by alkyl radical initiated linoleic acid oxidation system and diphenyl bitter acid-hydrazide (DPPH) free radical system. Results the results of in vivo experiments showed that the activity of SOD in liver and thymus of rats decreased significantly after exposure to 0.3Gy of accumulated dose of low dose ionizing radiation, the activity of GSH-Px in serum, liver and spleen decreased significantly, and the activity of GSH-Px in serum and liver decreased significantly. The content of MDA in spleen was significantly increased. Cimetidine could increase the activity of antioxidant enzymes and decrease the content of MDA in serum and organs. The results showed that cimetidine could scavenge superoxide anion radical, hydroxyl radical, alkyl radical and DPPH radical in certain concentration range, and the scavenging ability of cimetidine to hydroxyl radical was the best. Conclusion cimetidine can effectively scavenge free radicals, which can reduce the oxidative stress induced by low dose cumulative irradiation by enhancing the activity of antioxidant enzymes and reducing the content of lipid peroxidation products in rats.
【作者单位】：上海海洋大学食品学院;海军医学研究所舰艇特殊环境生物效应与医学防护实验室;
【基金】：国家科技重大专项课题(2004ZX09J14103-07B)~~
【分类号】：R965

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