超高效液相色谱质谱联用法测定儿童血浆中利福平、利福喷丁和利福布汀的浓度研究

发布时间：2018-06-27 01:10

本文选题：利福平 + 利福喷丁　；参考：《中国临床药理学杂志》2015年21期

【摘要】：目的建立超高效液相色谱质谱联用法(UPLC-MS/MS)同时测定儿童血浆中利福平、利福喷丁和利福布汀的浓度方法。方法以扎来普隆为内标,血浆样品经乙腈沉淀蛋白处理后检测。ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm)为分析柱,以乙腈-水(15 mmol·L-1甲酸铵-0.05%甲酸)为流动相,梯度洗脱,用电喷雾离子化源(ESI),以正离子多反应监测(MRM),检测专属性、标准曲线与定量下限、精密度与回收率、基质效应和稳定性。结果利福平、利福喷丁和利福布汀的线性范围分别为100~5000,20~2000,20~2000 ng·m L-1。日内、日间精密度相对标准偏差(RSD)均小于15%。结论本法灵敏、简便、准确,可同时对利福平、利福喷丁及利福布汀进行治疗药物监测。
[Abstract]:Objective to establish a high performance liquid chromatography-mass spectrometry (UPLC-MS / MS) method for simultaneous determination of rifampicin, rifampicin and rifambutin in children's plasma. Methods Zaleplon was used as the internal standard, the plasma sample was detected by acetonitrile precipitation protein treatment. The chromatographic column (2.1 mm 脳 100mm ~ 1.8 渭 m) of UPLC HSS in plasma was used as the analytical column, and acetonitrile-water (15 mmol L-1 ammonium formate -0.05% formic acid) was used as the mobile phase, and the gradient elution was performed. Electrospray ionization source (ESI), positive ion multireaction monitoring (MRM), detection specificity, standard curve and quantitative lower limit, precision and recovery, matrix effect and stability. Results the linear range of rifampicin, rifapentine and rifambutin was 100 ~ 5000m ~ (-1) ~ 20 ~ 200ng / L ~ (-1). Within days, the relative standard deviation (RSD) of inter-day precision was less than 15. Conclusion this method is sensitive, simple and accurate, and can be used to monitor rifampicin, rifampicin and rifambutin simultaneously.
【作者单位】：河北省人民医院药学部;河北省胸科医院药学部;
【基金】：河北省卫计委课题基金资助项目(ZD20140491)
【分类号】：R96;O657.63

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