壳聚糖创面喷雾剂处方筛选及对大鼠创伤愈合作用的研究

发布时间：2018-06-27 21:52

  本文选题：壳聚糖 + 处方筛选　； 参考：《成都医学院学报》2016年01期

【摘要】：目的筛选壳聚糖伤口护理膜处方,并评价其促进创伤愈合的效果。方法采用酸碱性、透气性和成膜性为评价指标,L9(34)正交表进行正交试验,筛选出壳聚糖创面喷雾剂最佳处方。制备SD大鼠创伤模型,以存活率、创面愈合时间、创面愈合率评价其促进伤口愈合的能力。结果筛选出的最佳处方组成为:壳聚糖0.1g、乳酸0.05mL、水20mL和乙醇10mL。实验组、对照组和空白组恢复创伤当天体质量的时间分别为(7.14±1.21)d、(7.00±1.15)d和(10.00±2.45)d,与空白组相比差异均有统计学意义(P=0.018和P=0.013);实验组、对照组和空白组创面愈合时间分别为(11.17±0.75)d、(12.33±0.82)d和(14.00±0.89)d,与空白组相比差异均有统计学意义(P=0.012和P=0.038);与空白组相比,实验组和对照组在伤后各时间点的创面愈合率均明显提高(P0.05)。结论自制壳聚糖创面喷雾剂能明显缩短伤口愈合时间,促进创面愈合。
[Abstract]:Objective to screen the prescription of chitosan wound nursing membrane and evaluate its effect on wound healing. Methods orthogonal test was carried out with acid and base, air permeability and film forming index, and the best formulation of chitosan wound spray was obtained. SD rat trauma model was established to evaluate its ability to promote wound healing in terms of survival rate, wound healing time and wound healing rate. Results the optimum prescription was: chitosan 0.1 g, lactic acid 0.05 mL, water 20 mL and ethanol 10 mL. The time to recover the body mass of the experimental group, control group and blank group were (7.14 卤1.21) d, (7.00 卤1.15) d and (10.00 卤2.45) d, respectively, which were significantly different from those of the blank group (P0. 018 and P0. 013). The wound healing time of the control group and the blank group were (11.17 卤0.75) d, (12.33 卤0.82) d and (14.00 卤0.89) d, respectively, which were significantly different from those of the blank group (P0. 012 and P0. 038). Conclusion chitosan spray can shorten wound healing time and promote wound healing.
【作者单位】： 成都医学院药学院;成都中医药大学;
【基金】：省级大学生创新创业训练计划项目(No:201313705015) 成都医学院“大学生创新实验计划”(No:CXXS201320) 四川省教育厅重点项目(No:14ZA0232)
【分类号】：R965
，

本文编号：2075400