内酯型双乙酰槐糖脂对肿瘤细胞K562的抑制作用

发布时间：2018-07-05 02:52

本文选题：内酯型双乙酰槐糖脂 + 抗肿瘤　；参考：《应用与环境生物学报》2015年02期

【摘要】：槐糖脂是一类由酵母菌产生的糖脂类生物表面活性剂.双乙酰化的内酯型槐糖脂具有很好的药物学活性,研究其抗肿瘤活性对于拓宽槐糖脂的应用领域具有重要意义.通过MTT法倒置相差显微镜观察、荧光染色、DNA电泳研究内酯型双乙酰槐糖脂对肿瘤细胞K562的抑制作用.结果显示:槐糖脂能够抑制肿瘤细胞K562的增殖,作用24 h和48 h,IC50值分别为31.70μg/m L和29.80μg/m L.细胞形态发生了明显的变化,细胞胀大,细胞膜破裂,细胞内容物外溢、细胞解体.细胞核出现收缩、碎裂和溶解.DNA发生随机断裂,电泳时出现弥散性涂抹状条带.本研究表明,内酯型双乙酰槐糖脂能够抑制肿瘤细胞K562的增殖,细胞坏死是抑制K562细胞增殖的主要原因.
[Abstract]:Sophora acacia glycolipid is a kind of biosurfactant which is produced by Saccharomyces cerevisiae. Bisacetylated acid-type locust glycolipid has good pharmacological activity, so it is important to study its antitumor activity for broadening the application field of locust glycoresin. The inhibitory effect of lactone diacetyl locust glycolipid on K562 cell line was studied by MTT method and fluorescence staining DNA electrophoresis. The results showed that saccharin could inhibit the proliferation of K562 cells. The IC50 of K562 cells was 31.70 渭 g / mL and 29.80 渭 g / mL at 24 h and 48 h, respectively. There were obvious changes in cell morphology, cell expansion, cell membrane rupture, overflow of cell contents and cell disintegration. Nuclear contraction, fragmentation and dissolution of DNA were observed at random, and diffuse smear bands appeared in electrophoresis. This study showed that lactone diacetyl locust glycolipid could inhibit the proliferation of K562 cells, and cell necrosis was the main reason for inhibiting the proliferation of K562 cells.
【作者单位】：山东大学生命科学学院;齐鲁工业大学山东微生物工程重点实验室;
【基金】：山东大学博士后日常基金和齐鲁工业大学博士基金资助~~
【分类号】：R96

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