川芎嗪注射液与阿司匹林和氯吡格雷联用抗家兔血栓形成的作用

发布时间：2018-07-08 21:36

本文选题：川芎嗪 + 体外血栓形成　；参考：《中国临床药理学杂志》2017年07期

【摘要】：目的研究川芎嗪注射液与阿司匹林、氯吡格雷联用抗家兔血栓形成的作用。方法按照体重将健康日本大耳白兔随机分为7组:空白组对照A、B组,低、高2个剂量中药对照组、联合A、B组,每组6只。空白组给予等容积0.9%NaCl,对照A组给予阿司匹林5.13 mg·kg~(-1),对照B组给予氯吡格雷3.85mg·kg~(-1),低、高2个剂量(川芎嗪注射液6.54,26.16 mg·kg~(-1))中药对照组,联合A、B组(中药对照高剂量组分别加对照A、B组)。川芎嗪注射液耳缘静脉给药10 d以及阿司匹林、氯吡格雷灌胃给药7 d在最后1天给药1 h后,心脏取血,通过体外血栓形成仪测定血栓长度、湿重;通过血小板聚集仪测定在血小板活化因子(PAF)诱导下对血小板聚集率的影响;通过酶联免疫吸附试剂盒测定血清血小板活化因子(PAF)、P-选择素、血小板因子-4(PF-4)的含量。结果联合B组的血栓长度为(7.4±4.6)cm,与空白组的(22.8±2.3)cm比较,差异有统计学意义(P0.01)。对照A、B组和高剂量中药对照组及联合A、B组的血栓湿重分别为(0.31±0.04),(0.30±0.05),(0.35±0.14),(0.28±0.08),(0.16±0.07)g,与空白组的(0.55±0.10)g比较,差异有统计学意义(均P0.01)。在PAF诱导下,低、高2个剂量中药对照组、联合A、B组的血小板聚集率分别为(43.3±4.7)%,(37.2±4.7)%,(35.5±4.1)%,(35.0±3.2)%与空白组的(52.9±4.4)%比较,差异有统计学意义(均P0.01)。与低剂量中药对照组比较,联合A、B组血小板聚集率明显降低,差异有统计学意义(P0.05)。低、高2个剂量中药对照组、联合A、B组的PAF含量分别为(1043±162),(1010±189),(973±159),(959±178)ng·L~(-1),与空白组的(1510±319)ng·L~(-1)比较,差异有统计学意义(均P0.01)。对照A、B组,低、高2个剂量中药对照组、联合A、B组的P-选择素含量分别为(156±13),(130±21),(101±17),(85±8),(107±18),(107±19)ng·mL~(-1),与空白组的(234±69)ng·mL~(-1)比较,差异有统计学意义(P0.01)。对照A、B组和高剂量中药对照组及联合A、B组的PF-4含量分别为(148±14),(255±14),(322±69),(242±26),(266±36)ng·mL~(-1),与空白组的(478±91)ng·mL~(-1)比较,差异有统计学意义(P0.05,P0.01)。结论川芎嗪注射液与阿司匹林、氯吡格雷联用能起到协同抗血栓形成的作用。
[Abstract]:Objective to study the antithrombotic effect of ligustrazine injection combined with aspirin and clopidogrel in rabbits. Methods healthy Japanese white rabbits were randomly divided into 7 groups according to their body weight: control group (control group), control group (n = 6), control group (n = 2) with low and high doses of traditional Chinese medicine and group A (n = 6). The blank group was given equal volume 0.9NaCl1, the control group was given aspirin 5.13 mg kg ~ (-1), the control group B was given clopidogrel 3.85mg ~ (-1), the dosage was low and high (Ligustrazine injection 6.54 ~ 26.16 mg 路kg ~ (-1) in the Chinese medicine control group, and the combination group (Chinese medicine control group with high dose group plus control group AHB group). Ligustrazine injection was given orally for 10 days and aspirin. Clopidogrel was given intragastrically for 7 days. After 1 hour of administration on the last day, the blood was taken from the heart, and the length and wet weight of the thrombus were measured by external thrombogenesis instrument. The effect of platelet activating factor (PAF) on platelet aggregation was determined by platelet aggregation assay, and the contents of P- selectin and platelet factor-4 (PF-4) in serum were measured by enzyme linked immunosorbent assay (Elisa). Results the thrombus length of combined group B was (7.4 卤4.6) cm, which was significantly higher than that of control group (22.8 卤2.3) cm (P0.01). The wet weight of thrombus was (0.31 卤0.04), () 0.30 卤0.04), (0.35 卤0.14), (0.28 卤0.08), (0.16 卤0.07) g in control group, high dose group (0.55 卤0.10) g and control group (0.55 卤0.10) g, respectively (P0.01). Under the induction of PAF, the platelet aggregation rates were (43.3 卤4.7), (37.2 卤4.7), (35.5 卤4.1)% and (35.0 卤3.2)% in the low and high dose Chinese medicine control group respectively, and the difference was statistically significant compared with the blank group (52.9 卤4.4)% (all P 0.01). The platelet aggregation rates of the two groups were (43.3 卤4.7), (37.2 卤4.7), (35.5 卤4.1)% and (52.9 卤4.4)%, respectively. Compared with the control group of low dose of Chinese medicine, the platelet aggregation rate of the combined group was significantly lower than that of the control group (P0.05). The contents of PAF in the low and high dose group were (1043 卤162), (, 1010 卤189), (, 959 卤178) ng L ~ (-1), which were significantly higher than those in the control group (1510 卤319) ng L ~ (-1) (all P 0.01). The P- selectin content in the control group (156 卤13), (, 130 卤21), (卤21), (, 101 卤8), (, 107 卤18), (107 卤19 ng ML-1) was significantly higher than that in the control group (234 卤69 ng mL ~ (-1), P 0.01). The content of PF-4 in the control group (148 卤14), (, 255 卤14), (, 322 卤69), (, 242 卤26), (, 266 卤36) ng mL ~ (-1) was significantly higher than that in the control group (478 卤91) ng mL ~ (-1), compared with the blank group (478 卤91) ng / mL ~ (-1), P 0.01). Conclusion Ligustrazine injection combined with aspirin and clopidogrel can play a synergistic role in anti-thrombosis.
【作者单位】：宜春学院化学与生物工程学院;
【基金】：国家自然科学基金-地区科学基金资助项目(81560584)
【分类号】：R965

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