以PTP1B为靶点的降血糖药用真菌的筛选

发布时间：2018-07-12 10:25

本文选题：蛋白质酪氨酸磷酸酶-B + 药用真菌　；参考：《生物技术》2016年06期

【摘要】：[目的]以胰岛素信号通路中负调节蛋白PTP1B为靶点,筛选降糖药用真菌。[方法]比较了蛹虫草、蝙蝠蛾拟青霉、桑黄、灰树花4种药用真菌粗提物对PTP1B的抑制活性,并通过糖尿病小鼠模型进行验证。[结果]蝙蝠蛾拟青霉对PTP1B的抑制率最高,达到(78±2.81)%,其抑制类型为竞争性抑制。体内实验显示,蝙蝠蛾拟青霉降血糖效果最佳,小鼠空腹血糖降低了71.2%,与模型组有显著差异(p0.01),和二甲双胍组相近。蛹虫草组、灰树花组和桑黄组血糖分别降低了62.7%、57.8%和48.3%。此外,各个实验组小鼠的甘油三酯、胆固醇、糖化血红蛋白和胰岛素水平与模型组也存在显著差异。[结论]PTP1B作为糖尿病的新靶标,在筛选降糖药用真菌的过程中也同样适用,而且蝙蝠蛾拟青霉显示了良好的降血糖活性。
[Abstract]:[objective] to screen hypoglycemic medicinal fungi using PTP1B, a negative regulatory protein in insulin signaling pathway. [methods] the inhibitory activities of crude extracts of four medicinal fungi, Cordyceps militaris, Paecilomyces dauricola, mulberry, and Grifola frondosa on PTP1B were compared and verified by diabetic mouse model. [results] the inhibitory rate of Penicillium dauricola on PTP1B was the highest (78 卤2.81), and its inhibition type was competitive inhibition. In vivo experiments showed that Paecilomyces dauricola had the best hypoglycemic effect, and the fasting blood glucose in mice decreased by 71.2%, which was significantly different from that in the model group (p0.01), and was similar to that in the metformin group. The blood glucose of Cordyceps militaris group, Grifola frondosa group and mulberry yellow group were decreased by 57.8% and 48.3%, respectively. In addition, the levels of triglyceride, cholesterol, glycosylated hemoglobin and insulin in each experimental group were significantly different from those in the model group. [conclusion] PTP1B, as a new target for diabetes mellitus, is also suitable for screening hypoglycemic medicinal fungi, and Paecilomyces dauricola shows good hypoglycemic activity.
【作者单位】：吉林医药学院;
【基金】：吉林市科技发展计划资助项目(“蛹虫草发酵菌粉产品的开发”,No.20156430)
【分类号】：R977.15

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