非诺贝特抑制人胃癌MGC803细胞增殖及机制

发布时间：2018-07-15 13:07

【摘要】：目的:探讨非诺贝特对胃癌MGC803细胞生物活性的影响及作用机制。方法:采用MTT法检测非诺贝特对胃癌MGC803细胞的增殖抑制作用;采用流式细胞术观察非诺贝特对MGC803细胞周期和凋亡的影响;免疫荧光染色检测非诺贝特对MGC803细胞Bcl-2/Bax蛋白表达及细胞生长的抑制作用;蛋白免疫印迹法(Western Blotting)检测非诺贝特对MGC803细胞p-ERK1/2、p-AKT、ERK1/2和AKT蛋白表达的影响。结果:非诺贝特对MGC803细胞增殖抑制作用具有时间和剂量依赖性。非诺贝特使MGC803细胞的周期阻滞于G2/M期,并诱导其凋亡,Bcl-2表达逐渐减少,Bax表达逐渐增加,且下调ERK1/2和AKT蛋白的磷酸化水平,但对ERK1/2和AKT蛋白表达水平没有影响。结论:非诺贝特能够抑制胃癌MGC803细胞增殖,并诱导其凋亡。其分子机制可能包括下调ERK1/2和AKT蛋白的磷酸化水平。
[Abstract]:Aim: to investigate the effect of fenofibrate on the biological activity of gastric cancer MGC803 cells and its mechanism. Methods: MTT assay was used to detect the inhibitory effect of fenofibrate on the proliferation of gastric cancer MGC803 cells, flow cytometry was used to observe the effect of fenofibrate on the cell cycle and apoptosis of MGC803 cells. The inhibitory effect of fenofibrate on Bcl-2 / Bax protein expression and cell growth in MGC803 cells was detected by immunofluorescence staining, and the effect of fenofibrate on the expression of p-ERK1 / 2p-AKTERK1 / 2 and AKT protein in MGC803 cells was detected by Western blotting. Results: fenofibrate inhibited the proliferation of MGC803 cells in a time and dose dependent manner. Fenofibrate blocked MGC803 cell cycle in G 2 / M phase and induced apoptosis of MGC803 cells. The expression of Bcl-2 decreased gradually and the expression of Bax increased gradually, and the phosphorylation level of ERK1 / 2 and AKT protein was down-regulated, but it had no effect on the expression of ERK1 / 2 and AKT protein. Conclusion: fenofibrate can inhibit the proliferation and induce apoptosis of gastric cancer MGC803 cells. Its molecular mechanism may include down-regulation of phosphorylation levels of ERK1 / 2 and AKT proteins.
【作者单位】：天津市泰达医院药剂科;黑龙江省医院药剂科;
【分类号】：R96

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