HPLC测定绿茶浸出液灌胃动脉粥样硬化大鼠血浆中4个有效成分的含量

发布时间：2018-07-26 18:34

【摘要】：目的:建立HPLC测定绿茶浸出液中没食子酸、咖啡因、表儿茶素没食子酸酯、山柰酚在动脉粥样硬化大鼠体内的血药浓度,为动脉粥样硬化的治疗提供参考。方法:动脉粥样硬化大鼠灌胃绿茶浸出液后,分别在不同时间点取血,采用依利特Hypersil ODS2色谱柱(4.6 mm×250 mm,5μm),流动相甲醇(A)-0.03%磷酸水溶液(B)梯度洗脱(0~30 min,10%~43%A;30~43 min,43%~45%A;43~44 min,45%~51%A;44~60 min,51%~53%A;60~70 min,53%~90%A),流速0.7 mL·min~(-1),检测波长278 nm的色谱条件进样分析。结果:没食子酸、咖啡因、表儿茶素没食子酸酯、山柰酚分别在0.255~20.40,0.430~86.00,0.245~19.60,0.270~21.60 mg·L~(-1)内呈良好线性关系,日内和日间精密度RSD均9.0%,提取回收率和方法回收率都87%;酸化后主要药代动力学参数药时曲线下面积(AUC0-t)分别为(8.902±1.235),(198.5±12.5),(7.367±0.412),(4.426±0.251)mg·h·L~(-1),表观分布容积(Vd)分别为(0.990±0.134),(1.409±0.201),(0.501±0.243),(0.029±0.009)L·kg~(-1),达峰时间(T_(max))均为1.5 h。结论:该方法对所测成分的分离度好,适用于测定绿茶浸出液中4个有效成分的血药浓度,为临床预防此类疾病提供一定理论依据。
[Abstract]:Objective: to establish a HPLC method for the determination of Gallic acid, caffeine, epigallocatechin gallate and kaempferol in rats with atherosclerosis, and to provide reference for the treatment of atherosclerosis. Methods: rats with atherosclerotic rats were treated with green tea extract, and blood samples were collected at different time points. The mobile phase methanol (A) -0.03% phosphoric acid solution (B) gradient elution (0.30 min) was used with the column of 4.6 mm 脳 250mm-1 5 渭 m), methanol (A) -0.03% phosphoric acid solution. The chromatographic conditions were analyzed at 278nm. The flow rate was 0.7 mL min ~ (-1) and the flow rate was 0.7 mL 路min ~ (-1) at the flow rate of 0.7 mL 路min ~ (-1) and the flow rate was 0.7 mL 路min ~ (-1). The chromatographic conditions were analyzed at the wavelength of 278nm. The chromatographic conditions were analyzed at the wavelength of 278nm. The chromatographic conditions were as follows: (1) the flow rate was 0. 7 mL 路min ~ (-1) and the flow rate was 0. 7 mL 路min ~ (-1). Results: Gallic acid, caffeine, epigallocatechin gallate and kaempferol showed a good linear relationship in the range of 0.255U 20.400.4030C 86.00 0.245L ~ (19.60) ~ (0.270) mg / L ~ (-1), and 0.270mg / L ~ (-1), respectively. The area under curve (AUC0-t) of the main pharmacokinetic parameters after acidification was (8.902 卤1.235), (卤12.5), () 7.367 卤0.412), (4.426 卤0.251) mg h L ~ (-1), and the apparent distribution volume (Vd) was (0.990 卤0.134), (1.409 卤0.201), (0.501 卤0.243), (0.029 卤0.009) L kgL ~ (-1), the peak time (T _ (max) was 1.5 h. Conclusion: this method is suitable for the determination of serum concentration of four active components in green tea extract and provides a theoretical basis for clinical prevention of this kind of disease.
【作者单位】：贵州医科大学医学检验学院;贵州师范大学分析测试中心;
【基金】：国家自然科学基金地区科学基金项目(81560720) 喀斯特地区生物与信息技术协同创新中心项目(黔教合协同创新字[2014]04)
【分类号】：R965

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