固态培养对产紫杉醇真菌小孢拟盘多毛孢次级代谢物生成影响

发布时间：2018-07-26 18:17

【摘要】：激活真菌基因组中沉默基因簇,可令其产生新的次生代谢产物,改变培养条件是有效的激活方法.本文研究了固态培养方法对一株产抗癌药物紫杉醇(Taxol)真菌——小孢拟盘多毛孢(Pestalotiopsis microspora)次级代谢产物生成的影响.实验发现固体发酵能够产生2种液体发酵中没有的产物,对其中一种产物进行了高效液相色谱(HPLC)分离纯化和定量分析.该物质的最高产量达到每克培养基质0.354mg,在HPLC谱上的保留时间和紫杉醇接近,值得进一步研究其结构和生物学活性.近年来,固体发酵技术以其独特性和环保优势,再次受到重视.本实验显示固态培养能够诱导该真菌产生2种稳定的次生代谢物,比单纯液体发酵更有优势.本研究还优化了固体发酵基质、发酵时间等条件,为进一步后续研究提供参考.
[Abstract]:Activation of silencing gene clusters in fungal genomes can produce new secondary metabolites. Changing culture conditions is an effective activation method. The effect of solid state culture on the production of secondary metabolites of (Pestalotiopsis microspora), a taxol-producing (Taxol) fungus, was studied. It was found that solid fermentation could produce two kinds of products which were not found in liquid fermentation. One of the products was separated and purified by high performance liquid chromatography (HPLC) and analyzed quantitatively. The maximum yield of the compound reached 0.354mg per gram of culture substrate, and the retention time in HPLC spectra was close to that of paclitaxel, which was worthy of further study on its structure and biological activity. In recent years, solid fermentation technology has been paid more attention for its unique and environmental advantages. The results showed that solid culture could induce the fungus to produce two stable secondary metabolites, which was superior to liquid fermentation alone. This study also optimized the solid fermentation substrate, fermentation time and other conditions to provide a reference for further research.
【作者单位】：北京师范大学生命科学学院;
【基金】：国家高技术研究发展计划((863)资助项目(2012AA023405))
【分类号】：R915

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