大鼠中抗凝血化合物TI-02的含量测定研究
发布时间：2018-07-31 12:11
【摘要】：对一类肽化合物的抗凝活性进行初步筛选,同时建立测定大鼠血浆中化合物TI-02浓度的HPLC方法。选用C18柱(250 mm×4.6 mm,10μm),采用甲醇-乙腈-水三元体系梯度洗脱,流速1.0 mL·min~(-1),检测波长240 nm,柱温30℃,进样量20μL。抗凝血化合物TI-02在浓度0.125~10.0μg/mL范围内线性良好,r=0.9964;回收率为97.1%~106.7%;日内、日间精密度RSD2.3%,室温24 h内稳定,RSD1.0%。本实验建立HPLC测定方法灵敏度高、精密度好,结果稳定可靠,可用于该肽类化合物大鼠血浆中浓度的测定。
[Abstract]:The anticoagulant activity of a class of peptide compounds was preliminarily screened and a HPLC method for the determination of TI-02 in rat plasma was established. C _ (18) column (250mm 脳 4.6mm ~ (10) 渭 m),) was used for gradient elution of methanol-acetonitrile-water ternary system with a flow rate of 1.0 mL / min ~ (-1), detection wavelength of 240nm, column temperature of 30 鈩，
本文编号：2155547
[Abstract]:The anticoagulant activity of a class of peptide compounds was preliminarily screened and a HPLC method for the determination of TI-02 in rat plasma was established. C _ (18) column (250mm 脳 4.6mm ~ (10) 渭 m),) was used for gradient elution of methanol-acetonitrile-water ternary system with a flow rate of 1.0 mL / min ~ (-1), detection wavelength of 240nm, column temperature of 30 鈩，
本文编号：2155547
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