黄芩苷联合美罗培南对小鼠腹腔铜绿假单胞菌早期生物被膜的体内影响

发布时间：2018-08-02 14:53

【摘要】：目的构建小鼠腹腔铜绿假单胞菌(PAE)早期生物被膜(BF)感染模型,探讨黄芩苷(Baicalin)联合美罗培南(MEM)对PAE体内早期BF感染的作用。方法以输液管为载体,PAO1野生株为实验菌株,32只雌性小鼠随机分为空白对照组、Baicalin组(60mg/Kg·d)、MEM组(50mg/Kg·d)、Baicalin(60mg/Kg·d)+MEM(50mg/Kg·d)组,建立小鼠腹腔BF感染模型后开始腹腔注射上述药物,空白对照组用等量无菌生理盐水;药物作用24h后行连续稀释法载体表面活菌计数,扫描电子显微镜(SEM)观察载体表面BF形态变化。结果药物干预24h,药物单独作用组或联合组载体表面菌落计数均少于空白对照组(P0.01);MEM组较Baicalin组减少(P0.01);Baicalin+MEM组均较Baicalin组、MEM组明显减少(P0.01);空白对照组SEM观察载体表面细菌均比Baicalin组、MEM组、Baicalin+MEM组形成的BF多而稠厚;MEM组较Baicalin组稀薄;Baicalin+MEM组BF基本被破坏清除,仅见少量PAE细菌粘附。结论 Baicalin对小鼠腹腔PAE早期BF具有破坏作用,与MEM联合应用可显著增强其对BF的杀菌作用。
[Abstract]:Objective to establish an early biofilm (BF) infection model of Pseudomonas aeruginosa (PAE) in mice and to investigate the effect of baicalin (Baicalin) combined with meropenem (MEM) on early BF infection in PAE. Methods Thirty-two female mice were randomly divided into control group (60mg/Kg d), 50mg/Kg group (50mg/Kg d) and Baicalin group (60mg/Kg d) MEM (50mg/Kg d). The model of intraperitoneal BF infection in mice was established and the above drugs were injected intraperitoneally. The blank control group was treated with the same amount of aseptic saline. After 24 hours of treatment, continuous dilution method was used to count the living bacteria on the surface of the carrier, and the morphology of BF on the surface of the carrier was observed by scanning electron microscope (SEM). Results after 24 hours of drug intervention, the number of carrier surface bacteria in the drug alone group or combination group was less than that in the blank control group (P0.01). The number of bacteria on the carrier surface in the Baicalin group was significantly lower than that in the Baicalin group (P0.01), and the number of bacteria on the carrier surface in the blank control group was significantly lower than that in the Baicalin group (P0.01). The BF formed in Baicalin group was more than that in Baicalin group and thicker than that in Baicalin group. The BF of Baicalin group was basically destroyed and cleared. Only a small amount of PAE bacteria adhesion was observed. Conclusion Baicalin can destroy the early BF of PAE in mice, and combined with MEM can significantly enhance its bactericidal effect on BF.
【作者单位】：广西医科大学第一附属医院呼吸疾病研究所;
【基金】：国家自然科学基金资助项目(81260663)
【分类号】：R96

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