α-硫辛酸对高盐诱发的高血压大鼠头端延髓腹外侧区内氧化应激的抑制作用

发布时间：2018-08-02 14:53

【摘要】：目的研究α硫辛酸可否在高盐诱导的高血压的发生发展中通过降低延髓头端腹外侧(RVLM)线粒体中活性氧(ROS)的过量产生来缓解高血压反应。方法按照体重将雄性大鼠随机分到2组:正常盐饮食(0.3%NaCl)8周和高盐饮食8周(以8%NaCl诱导高血压模型)(均n=14)。分别再分2个亚组:实验组(高血压模型+α硫辛酸)、模型组(高血压模型+0.9%NaCl)、对照组(正常盐饮食+α硫辛酸)和空白对照组(正常盐饮食+0.9%NaCl,均n=7)。在实验结束时,监测各组大鼠动脉压的变化;用ELISA测定丙二醛(MDA)、超氧化物歧化酶(SOD)以及谷胱甘肽(GSH)等含量或活性。用高效液相色谱法检测血清去甲肾上腺素(NE)水平。结果给与α硫辛酸9周后,模型组与实验组的平均动脉压(MAP)分别为(160.2±10.9),(128.2±9.6)mm Hg,差异有统计学意义(P0.05)。模型组与实验组的MDA分别为(6.1±3.0),(9.8±3.2)μmol·g~(-1),实验组明显降低,差异有统计学意义(P0.05);这2组的SOD水平分别为(16.2±1.3),(31.9±2.8)U·mg~(-1);这2组的GSH分别为(2.0±0.4),(4.9±0.8)μmol·g~(-1),实验组均明显增高,差异均有统计学意义(均P0.05)。模型组与实验组的血清去甲肾上腺素分别为(458.9±6.1),(322.8±7.2)pg·mL~(-1),差异有统计学意义(P0.05)。结论长期补充α硫辛酸可通过提高抗氧化能力来缓解高血压反应。
[Abstract]:Objective to study whether 伪 -lipoic acid can alleviate hypertension response by reducing the excessive production of reactive oxygen (ROS) in mitochondria of ventrolateral medulla (RVLM). Methods male rats were randomly divided into two groups according to their body weight: normal salt diet (0.3%NaCl) for 8 weeks and high salt diet for 8 weeks (induced by 8%NaCl). The rats were divided into two subgroups: experimental group (hypertension model 伪 -lipoic acid), model group (hypertension model 0.9%NaCl), control group (normal salt diet 伪 -lipoic acid) and blank control group (normal salt diet 0.9NaCl3, both nong7). At the end of the experiment, the changes of arterial pressure were monitored, and the contents or activities of malondialdehyde (MDA),) superoxide dismutase (SOD) and glutathione (GSH) (GSH) were measured by ELISA. The level of noradrenaline (NE) in serum was determined by high performance liquid chromatography (HPLC). Results after 9 weeks of 伪 -lipoic acid administration, the mean arterial pressure (MAP) of model group and experimental group was (160.2 卤10.9), () 128.2 卤9.6) mm Hg, (P0.05). The MDA of the model group and the experimental group were (6.1 卤3.0), (9.8 卤3.2) 渭 mol g ~ (-1), significantly decreased in the experimental group (P0.05), the SOD levels of the two groups were (16.2 卤1.3), (31.9 卤2.8) U mg ~ (-1), and the GSH of the two groups were (2.0 卤0.4), () 4.9 卤0.8 渭 mol g ~ (-1), which were significantly higher in the experimental group (P0.05). The serum norepinephrine in model group and experimental group was (458.9 卤6.1), (322.8 卤7.2pg mL ~ (-1), the difference was statistically significant (P0.05). Conclusion long-term supplementation of 伪-lipoic acid can relieve hypertension by increasing antioxidant capacity.
【作者单位】：成都市第七人民医院神经内科;
【分类号】：R965

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