高效液相色谱法同时测定人血清中茶碱、二羟丙茶碱、多索茶碱的浓度

发布时间：2018-08-18 16:19

【摘要】：目的:建立人血清中茶碱、二羟丙茶碱和多索茶碱血药浓度同时测定的高效液相色谱法并应用于患者的个体化治疗。方法:采用蛋白沉淀法对血样进行预处理,以咖啡因为内标。色谱条件:Eclipse XDB-C18(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-水梯度洗脱,流速为1.0 ml·min-1,检测波长273 nm,柱温为30℃。结果:茶碱在1.47~47.00μg·ml-1范围内线性良好(r=0.998),二羟丙茶碱在1.28~40.80μg·ml-1范围内线性良好(r=0.999),多索茶碱在1.44~46.00μg·ml-1范围内线性良好(r=0.999)。茶碱、二羟丙茶碱和多索茶碱的平均绝对回收率均达到95%左右,平均相对回收率分别为100.34%~105.48%,97.09%~103.13%和97.61%~101.39%,日内和日间RSD均小于10%。结论:本方法简便、快速、灵敏,可用于茶碱、二羟丙茶碱和多索茶碱的临床血药浓度监测及药动学研究。
[Abstract]:Objective: to establish a high performance liquid chromatography (HPLC) for simultaneous determination of theophylline, dihydroxypropylline and doxofylline in human serum. Methods: blood samples were pretreated by protein precipitation method and coffee was used as internal standard. The chromatographic conditions were as follows: eclipse XDB-C18 (4.6 mm 脳 150 mm ~ 5 渭 m) column, methanol-water gradient elution as mobile phase, flow rate 1.0 ml min-1, detection wavelength 273 nm, column temperature 30 鈩，

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