二咖啡酰奎宁酸对脑缺血后星形胶质细胞Porimin指标的影响观察

发布时间：2018-08-20 12:20

【摘要】：目的观察二咖啡酰奎宁酸对脑缺血后星形胶质细胞Porimin指标的影响。方法 32只雄性SD健康清洁级大鼠,分为空白组、对照组及药物组。应用大脑中动脉阻塞模型(MCAO)在对照组和药物组诱导脑缺血发作后,在对照组中予生理盐水,在药物组中予二咖啡酰奎宁酸。在MCAO后6h、24h分别进行行为学神经功能评分,HE染色、Cresyl Violet染色和GFAP、Porimin、DAPI免疫荧光染色。结果对照组和药物组中HE染色及Cresyl Violet均显示缺血中心区脑组织坏死,缺血半暗带区细胞水肿,可见肿胀的星形胶质细胞,对照组细胞损伤重于药物组。免疫荧光检测示药物组Porimin与GFAP、DAPI三标阳性细胞数目显著低于对照组(P=0.01)。结论二咖啡酰奎宁酸能降低脑缺血后星形细胞porimin指标的表达,提示二咖啡酰奎宁酸在脑缺血时对星形胶质细胞起一定积极的保护作用。
[Abstract]:Objective to observe the effect of dicaffeyl quinine on Porimin index of astrocytes after cerebral ischemia. Methods 32 healthy and clean male SD rats were divided into blank group, control group and drug group. The model of middle cerebral artery occlusion (MCAO) was used to induce cerebral ischemic attack in control group and drug group, then normal saline was given in control group, and dicaffeyl quinine was given in drug group. The behavioral neurologic function score (HE) and Cresyl Violet staining and GFAPP Porimintio DAPI immunofluorescence staining were performed at 6 h and 24 h after MCAO, respectively. Results HE staining and Cresyl Violet in the control group and drug group showed necrosis of brain tissue, edema and swelling of astrocytes in the ischemic penumbra. The damage of cells in the control group was more serious than that in the drug group. Immunofluorescence assay showed that the number of Porimin and GFAPP DAPI positive cells in the drug group was significantly lower than that in the control group (P0. 01). Conclusion dicaffeyl quinine can decrease the expression of porimin in astrocytes after cerebral ischemia, suggesting that dicaffeyl quinine may play an active role in the protection of astrocytes during cerebral ischemia.
【作者单位】：深圳市人民医院(暨南大学第二临床学院)神经内科;
【基金】：深圳市科技计划项目(201002004)
【分类号】：R96

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