香蕉果皮黄酮-丙酸复配剂的毒理学研究

发布时间：2018-08-20 16:18

【摘要】：本文采取微波提取法从香蕉皮中提取得到黄酮粗提物,利用H103大孔树脂对提取物进行纯化,与丙酸复配得到复配剂。以香蕉果皮黄酮-丙酸复配剂为受试物,根据国家卫生部发布的《食品安全性毒理学评价程序和方法》对其进行毒理学试验,以便在生产实践中推广应该。利用小鼠为试验对象,进行了急性毒性试验(LD50)、骨髓细胞微核试验、小鼠精子畸形试验以及哺乳动物微粒体酶试验(Ames试验),对复配剂的毒理性进行了研究。结果表明,雌小鼠LD50为10.80 g/kg BW,可信限为7.41~15.70 g/kg BW;雄小鼠LD50为12.60 g/kg BW,可信限为7.75~20.50 g/kg BW;骨髓细胞微核试验、精子畸形试验、Ames试验的结果都为阴性,表明香蕉果皮黄酮-丙酸复配剂对小鼠体细胞和生殖细胞无诱变作用,也无直接或间接的致突变作用,属实际无毒级物质。香蕉果皮黄酮-丙酸复配剂可作为食品防腐添加剂。
[Abstract]:In this paper, flavonoids were extracted from banana peel by microwave extraction. The extracts were purified by H103 macroporous resin and mixed with propionic acid. Based on the food safety toxicology evaluation procedure and method issued by the Ministry of Health, the flavone-propionic acid compound from banana peel was tested in toxicology in order to popularize it in production practice. Acute toxicity test (LD50), bone marrow micronucleus test, sperm malformation test and mammalian microsomal enzyme test (Ames test) were carried out in mice. The results showed that the LD50 of female mice was 10.80 g/kg BWand the confidence limit was 7.41 g/kg BW.The male mouse LD50 was 12.60 g/kg BWand the confidence limit was 7.750.50 g/kg BW.The results of bone marrow micronucleus test and sperm malformation test were all negative. The results showed that the compound of flavonoids and propionic acid in banana pericarp had no mutagenic effect on somatic cells and germ cells of mice, nor had direct or indirect mutagenicity. Banana peel flavone-propionic acid compound can be used as food preservative additive.
【作者单位】：广州大学化学化工学院食品系;
【基金】：广东省广州市科技计划项目(2013J4100020)
【分类号】：TS201.6;R994.4

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