Fas途径调控芬太尼与5-氟尿嘧啶联合诱导MCF-7乳腺癌细胞凋亡

发布时间：2018-08-21 09:15

【摘要】：目的探讨芬太尼与5-氟尿嘧啶(5-FU)联合应用对人乳头癌细胞株(MCF-7)乳腺癌细胞凋亡的影响及Fas途径的调控机制。方法作用48 h后通过流式细胞技术(FCM)检测细胞凋亡检测试剂盒/碘化丙啶(Annexin V-EGFP/PI)染色的细胞凋亡情况,通过Western印迹检测Fas、半胱氨酸蛋白酶(Caspase)-8表达情况。结果 1μmol/L及10μmol/L浓度的芬太尼单独应用均引起凋亡率增加,Fas、Caspase-8表达增强(P0.05,P0.01);5-FU单独应用后凋亡率亦明显增加,Fas、Caspase-8表达增强(P0.01);三种浓度芬太尼与5-FU联合应用均具有协同作用(P0.01)。Caspase-8与Fas表达变化呈显著正相关(r=0.877,P0.01)。结论一定浓度的芬太尼、5-FU及联合应用48 h对MCF-7乳腺癌细胞有促进凋亡作用,并显著上调Fas表达和Caspase-8活性,两药联合应用启动外源性凋亡途径具有协同作用。
[Abstract]:Objective to investigate the effect of fentanyl combined with 5-fluorouracil (5-FU) on apoptosis of human papillary carcinoma cell line (MCF-7) and the regulation of Fas pathway. Methods apoptosis was detected by flow cytometry (FCM) after 48 h, and the expression of Fas-8 and cysteine protease (Caspase) -8) was detected by Western blot. Results both 1 渭 mol/L and 10 渭 mol/L fentanyl alone could increase the apoptosis rate and increase the expression of Fas-Fas-Caspase-8 (P0.05-FU), and the combination of fentanyl and 5-FU could increase the expression of Caspase-8 (P0.01). Caspase-8 was a synergistic effect of fentanyl and 5-FU (P0.01), and the combination of Fentanyl and 5-FU had a synergistic effect (P0.01) .Caspase-8 expression was increased in combination with Fentanyl (P0.01) and Fentanyl at 10 渭 mol/L (P0.01). There was a significant positive correlation with the expression of Fas (r = 0.87 7, P 0.01). Conclusion Fentanyl 5-FU at a certain concentration and combined administration for 48 h can promote apoptosis of MCF-7 breast cancer cells and significantly up-regulate the expression of Fas and the activity of Caspase-8. The combination of Fentanyl and Fentanyl has synergistic effect on the initiation of exogenous apoptotic pathway.
【作者单位】：承德医学院附属医院麻醉科;平泉县杨树岭镇中心卫生院外科;
【分类号】：R96

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