药食用真菌代谢产物的活性研究
发布时间：2018-08-22 11:53
【摘要】：目的:研究药食用真菌的酿酒酵母抑制活性及抗氧化活性。方法:将实验用的药食用真菌进行分离纯化,用菌丝体进行液体培养,取其代谢产物进行酿酒酵母抑制活性试验,筛选具有该活性的菌株。再从活性菌株中分离出具有酿酒酵母抑制活性的化合物。采用DPPH法、Fenton法和邻苯三酚自氧化法对7种最为常见食用真菌不同部位提取物的抗氧化活性进行研究。结果:通过酿酒酵母抑制活性试验,筛选出了两种活性较好的的菌株,分别为鸡枞菌和BLG-3。从鸡枞菌代谢产物中分离出三种化合物,鉴定了其中两种为麦角甾醇和腺嘌呤核苷。麦角甾醇在4 mg/m L浓度下平均抑菌直径为11.7 mm,具有较好的酿酒酵母抑制活性。从BLG-3代谢产物中分离出一种化合物鉴定为胶霉毒素,其在4 mg/mL浓度下平均抑菌直径为12.6 mm,具有较好的酵母抑制活性。7种食用真菌提取物对DPPH都具有一定的清除能力,其中姬菇菌柄、茶树菇菌盖和香菇菌盖的提取物较其他样品的清除效果强;对羟基自由基除茶树菇菌盖提取物有一定的清除能力,其余提取物的清除能力较弱;对超氧阴离子除茶树菇菌盖较强,茶树菇菌柄和香菇菌盖有一定的清除能力外,其余提取物的清除效果较弱。结论:由于酿酒酵母是筛选抗肿瘤药物的良好模型,麦角甾醇和胶霉毒素对其有较好的抑制作用,因此这两种化合物在筛选抗肿瘤药物的过程中具有重要的研究价值;7种食用真菌不同部位提取物均具有一定的抗氧化活性,其中茶树菇菌盖综合抗氧化活性最佳。
[Abstract]:Objective: to study the inhibitory and antioxidation activities of edible fungi in Saccharomyces cerevisiae. Methods: the edible fungi used in the experiment were isolated and purified. The mycelium was used for liquid culture. The metabolites of the fungi were used for the inhibition test of Saccharomyces cerevisiae and the strains with the activity were screened. The compounds with inhibitory activity of Saccharomyces cerevisiae were isolated from the active strains. The antioxidant activities of extracts from different parts of seven most common edible fungi were studied by DPPH method and pyrogallol autooxidation method. Results: through the inhibitory activity test of Saccharomyces cerevisiae, two strains with better activity were screened out, which were fir and BLG-3, respectively. Three compounds were isolated from the metabolites of abieties, and two of them were identified as ergosterol and adenosine. The average inhibitory diameter of ergosterol at 4 mg/m / L concentration was 11.7mm, which showed that ergosterol had better inhibitory activity of Saccharomyces cerevisiae. A compound isolated from the metabolites of BLG-3 was identified as collotoxin with an average inhibitory diameter of 12.6mm at the concentration of 4 mg/mL. The extracts of mushroom stalk, tea mushroom and lentinus edodes had stronger scavenging effect than other samples, and had a certain scavenging ability to hydroxyl free radical, but the other extracts had weak scavenging ability. For superoxide anion, the scavenging effect of the other extracts was weak except for the strong tea mushroom cap, the tea mushroom stalk and the lentinus edodes cover. Conclusion: as Saccharomyces cerevisiae is a good model for screening antitumor drugs, ergosterol and collotoxin have a good inhibitory effect on it. Therefore, these two compounds have important research value in the process of screening anti-tumor drugs. The extracts of 7 edible fungi have certain antioxidant activities, among which the comprehensive antioxidant activity of tea mushroom mulches is the best.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R915
本文编号：2197019
[Abstract]:Objective: to study the inhibitory and antioxidation activities of edible fungi in Saccharomyces cerevisiae. Methods: the edible fungi used in the experiment were isolated and purified. The mycelium was used for liquid culture. The metabolites of the fungi were used for the inhibition test of Saccharomyces cerevisiae and the strains with the activity were screened. The compounds with inhibitory activity of Saccharomyces cerevisiae were isolated from the active strains. The antioxidant activities of extracts from different parts of seven most common edible fungi were studied by DPPH method and pyrogallol autooxidation method. Results: through the inhibitory activity test of Saccharomyces cerevisiae, two strains with better activity were screened out, which were fir and BLG-3, respectively. Three compounds were isolated from the metabolites of abieties, and two of them were identified as ergosterol and adenosine. The average inhibitory diameter of ergosterol at 4 mg/m / L concentration was 11.7mm, which showed that ergosterol had better inhibitory activity of Saccharomyces cerevisiae. A compound isolated from the metabolites of BLG-3 was identified as collotoxin with an average inhibitory diameter of 12.6mm at the concentration of 4 mg/mL. The extracts of mushroom stalk, tea mushroom and lentinus edodes had stronger scavenging effect than other samples, and had a certain scavenging ability to hydroxyl free radical, but the other extracts had weak scavenging ability. For superoxide anion, the scavenging effect of the other extracts was weak except for the strong tea mushroom cap, the tea mushroom stalk and the lentinus edodes cover. Conclusion: as Saccharomyces cerevisiae is a good model for screening antitumor drugs, ergosterol and collotoxin have a good inhibitory effect on it. Therefore, these two compounds have important research value in the process of screening anti-tumor drugs. The extracts of 7 edible fungi have certain antioxidant activities, among which the comprehensive antioxidant activity of tea mushroom mulches is the best.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R915
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