D-柠檬烯对酒精性脑损伤大鼠脑组织NF-κB、IκB及TNF-α表达的影响

发布时间：2018-08-22 14:02

【摘要】：目的观察D-柠檬烯对酒精性脑损伤大鼠脑组织NF-κB、IκB及TNF-α表达的影响。方法 60只Wistar大鼠随机分为6组,每组各10只。药物1、2、3组与模型组制作酒精性脑损伤模型,每日予50%乙醇灌胃,前2周为8 m L/(kg·d),后4周为12 m L/(kg·d),其中药物1、2、3组造模同时分别予100、200、400 mg/(kg·d)的D-柠檬烯灌胃;单纯药物组与空白对照组不制作动物模型,其中空白对照组每日予蒸馏水灌胃,单纯药物组在此基础上加400 mg/(kg·d)D-柠檬烯灌胃。实验第6周末处死动物,留取脑组织,HE染色,镜下观察其形态结构变化;免疫组化法测定脑组织中的NF-κB、IκB蛋白;ELISA双抗夹心法检测脑匀浆中的TNF-α。结果模型组神经元数量减少,分布不规则,细胞核明显固缩变形,药物1、2、3组脑组织形态结构变化逐渐减轻。模型组NF-κB阳性细胞数、IκB阳性细胞数与空白对照组相比,P均0.05。药物1、2、3组NF-κB阳性细胞数减少、IκB阳性细胞数增多,与模型组相比,P均0.05;药物3组与药物1、2组相比,P均0.05。模型组大鼠脑匀浆中TNF-α表达高于空白对照组(P均0.05);药物1、2、3组大鼠脑匀浆中TNF-α表达低于模型组(P均0.05),且药物3组低于药物1、2组(P均0.05)。结论 D-柠檬烯可降低酒精性脑损伤大鼠脑组织中NF-κB、TNF-α的表达,上调IκB的表达,进而减轻酒精性脑损伤。
[Abstract]:Objective to observe the effect of D-limonene on the expression of NF- 魏 B I 魏 B and TNF- 伪 in brain tissue of rats with alcoholic brain injury. Methods 60 Wistar rats were randomly divided into 6 groups with 10 rats in each group. The model of alcoholic brain injury was established in group 1 and group 2. The rats were given 50% ethanol daily, 12 mL / (kg d), for the first 2 weeks and 8 mL / (kg d), for 4 weeks. The rats in group 1 and 2 were given 100200400 mg/ (kg d) of D-limonene by stomach at the same time. There were no animal models in the drug group and the blank control group, in which the blank control group was fed with distilled water daily, and the simple drug group was fed with 400 mg/ (kg d) D-limonene. The animals were killed at the end of the 6th week of the experiment, the brain tissue was stained with HE and the changes of morphology and structure were observed under microscope, and the content of TNF- 伪 in brain homogenate was detected by Elisa double antibody sandwich method. Results in the model group, the number of neurons decreased, the distribution of neurons was irregular, the nucleus was obviously constricted and deformed. The number of NF- 魏 B positive cells and the number of I 魏 B positive cells in the model group were 0.05 compared with those in the blank control group. The number of NF- 魏 B positive cells in drug 1 + 2 + group decreased, the number of I 魏 B positive cells increased, compared with model group (P 0.05), drug 3 group (P < 0.05) compared with drug 1 "2" group (P < 0. 05). The expression of TNF- 伪 in the brain homogenate of the model group was higher than that in the blank control group (all P 0.05), and the expression of TNF- 伪 in the brain homogenate of the model group was lower than that in the model group (P 0.05), and the expression of TNF- 伪 in the brain homogenate of the drug group was lower than that in the control group (all P 0.05). Conclusion D- limonene can decrease the expression of NF- 魏 B TNF- 伪 and up-regulate the expression of I 魏 B in brain tissue of rats with alcoholic brain injury, and then attenuate the brain injury induced by alcohol.
【作者单位】：青岛大学公共卫生学院;青岛大学医学院附属青岛市市立医院;青岛大学基础学院细胞与分子生物学实验室;
【基金】：国家自然科学基金资助项目(31171671)
【分类号】：R965

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