HPLC法同时测定市售滴眼液中4种抑菌剂的含量

发布时间：2018-08-23 09:07

【摘要】：目的:建立同时测定市售滴眼液中羟苯甲酯、羟苯乙酯、羟苯丙酯和苯扎氯铵4种抑菌剂含量的方法。方法:采用高效液相色谱法。色谱柱为Hypersil GOLD C_(18),流动相为0.005 mol/L醋酸铵溶液(每1 L中含三乙胺10 mL,用冰醋酸调节p H为5.0±0.5)-乙腈(45∶55,V/V),流速为1.0 mL/min,检测波长分别为262 nm(羟苯甲酯、羟苯乙酯和羟苯丙酯)、214 nm(苯扎氯铵),柱温为30℃,进样量为20μL。结果:羟苯甲酯、羟苯乙酯、羟苯丙酯和苯扎氯铵检测质量浓度线性范围分别为1.235 0~15.438 0μg/mL(r=0.999 9)、1.317 0~16.383 6μg/mL(r=0.999 7)、1.207 2~15.089 4μg/mL(r=0.999 6)、17.776~222.0μg/mL(r=0.999 9);定量限分别为2.0、2.0、2.0、1.11μg,检测限分别为0.375、0.375、0.375、0.333μg;精密度、稳定性、重复性试验的RSD2.0%;加样回收率分别为98.14%~102.48%(RSD=1.6%,n=9)、98.79%~102.42%(RSD=1.3%,n=9)、98.19%~102.49%(RSD=1.5%,n=9)和98.76%~100.53%(RSD=0.6%,n=9)。结论:该方法结果准确、重复性好、操作简单,适用于市售滴眼液中羟苯甲酯、羟苯乙酯、羟苯丙酯和苯扎氯铵含量的同时测定。
[Abstract]:Objective: to establish a method for the simultaneous determination of methyl hydroxybenzoate, ethyl hydroxyl phenyl, hydroxypropyl ester and benzalkonium chloride in marketable eye drops. Methods: high performance liquid chromatography was used. The chromatographic column was Hypersil GOLD C _ (18), the mobile phase was 0.005 mol/L ammonium acetate solution (containing triethylamine 10 mL per 1 L), the pH was adjusted by glacial acetic acid (pH = 5.0 卤0.5) -acetonitrile (45: 55 V / V), the flow rate was 1.0 mL / min, and the detection wavelength was 262nm (hydroxybenzyl methyl ester). Hydroxyphenyl ethyl ester and hydroxypropyl ester) 214nm (benzalkonium chloride), the column temperature is 30 鈩，

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