星点设计-效应面法进行蛇床子素脂质体处方优化的研究

发布时间：2018-08-29 08:21

【摘要】：目的:以包封率为指标进行蛇床子素脂质体的处方优化研究,并对其质量进行考察。方法:以薄膜蒸发法制备蛇床子素脂质体。采用星点设计-效应面法优化处方,以包封率为因变量,大豆卵磷脂浓度、脂药比、表面活性剂含量为自变量建立多元二次回归方程,用效应面法预测较优处方并进行验证。结果:蛇床子素脂质体的多元二次回归方程为:Y1=0.935 69+0.010 124X1+0.038 691X3-0.024 32X21+0.025 741X1X2-0.054 236X22-0.026 579X2X3-0.109 707X23(r=0.953 7,P0.05),最佳处方分别为大豆卵磷脂浓度为24.93mg·m L-1,脂药比为22.66∶1(mg·mg-1),表面活性剂含量1.67%,该处方条件下,3批蛇床子素脂质体样品的平均包封率94.98%。结论:用星点设计-效应面法优选蛇床子素脂质体的制备工艺预测性好,制备的脂质体包封率高。
[Abstract]:Aim: to optimize the formulation and quality of osthol liposomes with encapsulation efficiency. Methods: osthol liposomes were prepared by thin film evaporation. The formula was optimized by star design-effect surface method. The multiple quadratic regression equation was established with the entrapment efficiency as dependent variable, soybean lecithin concentration, lipids / drug ratio and surfactant content as independent variables. The optimal formulation was predicted and verified by the effect surface method. Results: the multivariate quadratic regression equation of osthol liposome was: 1 Y 1C 0.935 69 0.010 124X1 0.038 691X3-0.024 32X21 0.025 741X1X2-0.054 236X22-0.026 579X2X3-0.109 707X23 (rr 0.953 7 741X1X2-0.054 236X22-0.026 579X2X3-0.109 707X23). The best formulation was 24.93mg ml -1, the ratio of lipids to lipids was 22.66: 1 (mg mg-1), and the surfactant content was 1.67%. The optimum formulation conditions were as follows: the concentration of soybean lecithin was 24.93mg ml -1, the ratio of lipid to drug was 22.66: 1 (mg mg-1), and the content of surfactant was 1.67%. The average entrapment efficiency of three batches of osthol liposomes was 94.98. Conclusion: the preparation process of osthol liposomes selected by star design-effect surface method is predictable and the entrapment efficiency of the prepared liposomes is high.
【作者单位】：广东药学院;广州中医药大学;
【分类号】：R943

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