耐酸性布拉氏酵母的筛选及高密度培养条件优化
发布时间：2018-08-30 13:50
【摘要】：布拉氏酵母菌(Saccharomyces boulardii)是临床上作为益生菌药物治疗肠道疾病使用的唯一一株酵母菌,作为微生态制剂,要保证其生物效果,必须能够在胃肠道中保持一定的活菌数,研究发现影响布拉氏酵母菌在胃肠道中存活的最主要因素是低p H,为提高布拉氏酵母菌在低p H条件下的存活率,该研究采用常压室温等离子体(ARTP)诱变技术对S.boulardii进行诱变,最终筛选出3株对低p H值耐受性较好的突变株。对突变株耐低p H稳定性的研究结果表明,在传代20次后,突变株YB-3具有较好的遗传稳定性,其存活率为51.79%。在5 L发酵罐中,对突变株YB-3高密度培养条件进行优化,最终所得布拉氏酵母菌体干质量为58.79 g/L,比原始菌株提高了55.52%。
[Abstract]:(Saccharomyces boulardii) is the only yeast used clinically as a probiotic drug for the treatment of intestinal diseases. As a microecological preparation, to ensure its biological effect, it must be able to maintain a certain number of live bacteria in the gastrointestinal tract. It was found that the most important factor affecting the survival of Saccharomyces brasilii in the gastrointestinal tract was low pH. In order to improve the survival rate of Saccharomyces brasiliensis at low pH, the S.boulardii was mutagenic by plasma (ARTP) at room temperature at atmospheric pressure. Finally, 3 mutants with good tolerance to low pH were selected. The results showed that the mutant YB-3 had good genetic stability after 20 passages, and its survival rate was 51.79%. In a 5L fermenter, the high density culture conditions of mutant YB-3 were optimized, and the dry mass of yeast was 58.79 g / L, which was 55.52% higher than that of the original strain.
【作者单位】： 天津科技大学生物工程学院工业发酵微生物教育部重点实验室;
【基金】：长江学者和创新团队发展计划资助(IRT1166)
【分类号】：R915
，
本文编号：2213267
[Abstract]:(Saccharomyces boulardii) is the only yeast used clinically as a probiotic drug for the treatment of intestinal diseases. As a microecological preparation, to ensure its biological effect, it must be able to maintain a certain number of live bacteria in the gastrointestinal tract. It was found that the most important factor affecting the survival of Saccharomyces brasilii in the gastrointestinal tract was low pH. In order to improve the survival rate of Saccharomyces brasiliensis at low pH, the S.boulardii was mutagenic by plasma (ARTP) at room temperature at atmospheric pressure. Finally, 3 mutants with good tolerance to low pH were selected. The results showed that the mutant YB-3 had good genetic stability after 20 passages, and its survival rate was 51.79%. In a 5L fermenter, the high density culture conditions of mutant YB-3 were optimized, and the dry mass of yeast was 58.79 g / L, which was 55.52% higher than that of the original strain.
【作者单位】： 天津科技大学生物工程学院工业发酵微生物教育部重点实验室;
【基金】：长江学者和创新团队发展计划资助(IRT1166)
【分类号】：R915
，
本文编号：2213267
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