卡托普利干预自发性高血压大鼠血清代谢组学研究

发布时间：2018-08-31 07:58

【摘要】：目的通过代谢组学方法研究卡托普利干预自发性高血压大鼠血清内源性代谢物的变化,进一步探索卡托普利的作用机制。方法采用快速高分离液相-四级杆飞行时间串联质谱联用技术采集大鼠血清内源性代谢物信息,经偏最小二乘法判别分析,识别显著差异的变量,鉴定潜在生物标志物。结果正常组、模型组、卡托普利组的血清内源性代谢物、代谢模式发生了明显变化。经数据库查询共确定了4个生物标记物及其代谢途径,与血管内皮功能密切相关。结论代谢组学从机体整体代谢角度揭示了卡托普利保护血管内皮功能的可能作用机制,在阐释药物复杂的作用机制方面显示出了独特的潜力。
[Abstract]:Objective to investigate the effects of captopril on the changes of serum endogenous metabolites in spontaneously hypertensive rats and to explore the mechanism of captopril. Methods the information of endogenous metabolites in rat serum was collected by high separation liquid-four-pole time-of-flight tandem mass spectrometry. By partial least squares discriminant analysis, significant differences were identified and potential biomarkers were identified. Results the serum endogenous metabolites and metabolic patterns of normal group, model group and captopril group were obviously changed. Four biomarkers and their metabolic pathways were identified by database query, which were closely related to vascular endothelial function. Conclusion Metabonomics reveals the possible mechanism of captopril in protecting vascular endothelial function from the point of view of whole body metabolism and shows unique potential in explaining the complex mechanism of drugs.
【作者单位】：山东中医药大学第一临床医学院;山东中医药大学实验中心;山东中医药大学附属医院高血压国家中药临床研究基地;
【基金】：中国博士后科学基金特别资助项目(No 2015T80741) 国家自然科学基金资助项目(No 81473653)
【分类号】：R965

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