精脒对SW620细胞增殖及糖酵解的影响

发布时间：2018-08-31 15:01

【摘要】：目的探讨精脒对人结肠癌SW620细胞增殖及糖酵解的影响。方法以SW620细胞为研究对象,用0.625~2.5μmo L/L精脒(SPD)作用于细胞,细胞密度以104个/m L接种于96孔培养板,分别培养24 h后,MTT法检测细胞增殖情况;同时,取6孔板培养24 h的细胞上清液,用试剂盒检测SW620细胞葡萄糖消耗及乳酸水平;细胞密度以1.2×10~5个/m L接种于6孔培养板中,培养24 h后加入不同浓度SPD,继续培养24 h后,Western blot检测缺氧诱导因子(HIF-1)、乳酸脱氢酶(LDHA)及葡萄糖转运蛋白1(GLUT1)表达的变化。结果与对照组比较,精脒各浓度组均能促进SW620细胞的增殖(P0.01),且促进作用呈剂量依赖性;精脒各浓度组作用后SW620细胞内葡萄糖的消耗及乳酸含量明显增多(P0.01),呈剂量依赖性;与对照组比较,LDHA、HIF-1及GLUT1蛋白表达水平随着精脒浓度的升高而增加(P0.01),且呈剂量依赖性。结论精脒具有促进人结肠癌SW620细胞增殖及糖酵解的作用。
[Abstract]:Objective to investigate the effect of spermidine on proliferation and glycolysis of human colon cancer SW620 cells. Methods SW620 cells were treated with 0.625 渭 mo L / L spermidine (SPD), and the cell density was 104 / mL inoculated on 96 well culture plate. After 24 h culture, cell proliferation was detected by MTT assay. The supernatant of 6-well plate cultured for 24 h was used to detect glucose consumption and lactic acid level of SW620 cells, the cell density was 1.2 脳 10 ~ 5 / mL and inoculated in 6-well culture plate. After 24 hours of culture, the expression of hypoxia inducible factor (HIF-1), lactate dehydrogenase (LDHA) and glucose transporter 1 (GLUT1) were detected by Western blot. Results compared with the control group, spermidine could promote the proliferation of SW620 cells (P0.01) in a dose-dependent manner, and the glucose consumption and lactic acid content in SW620 cells were increased in dose-dependent manner after the treatment of spermidine (P0.01). Compared with the control group, the expression of HIF-1 and GLUT1 protein increased with the increase of spermidine concentration (P0.01), in a dose-dependent manner. Conclusion spermidine can promote the proliferation and glycolysis of human colon cancer SW620 cells.
【作者单位】：广东药科大学药科学院;
【基金】：国家自然科学基金项目(81302895;81102465)
【分类号】：R96

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