苯肾上腺素对低氧肺损伤大鼠肺泡液体清除的作用

发布时间：2018-09-03 17:19

【摘要】：目的探讨α肾上腺素能受体激动剂苯肾上腺素对低氧肺损伤大鼠肺泡液体清除率(AFC)的作用及机制。方法低氧大鼠暴露于10%的氧浓度,分别于24 h和48 h测定AFC、总肺水量(TLW)。苯肾上腺素分别与α1受体阻滞剂哌唑嗪、α2受体阻滞剂育亨宾、β1受体阻滞剂阿替洛尔、β2受体阻滞剂ICI-118551、钠通道阻断剂氨氯吡咪及Na+-K+-ATP酶阻断剂哇巴因混合后灌注到低氧48 h大鼠的肺泡腔内,测定AFC的变化。结果大鼠暴露于10%的氧浓度24 h后AFC(17.09±2.36)%/h与正常大鼠AFC(16.85±2.87)%/h比较没有明显变化;48 h时AFC(8.41±2.67)%/h明显降低。苯肾上腺素能明显增加低氧大鼠AFC。苯肾上腺素增加低氧大鼠AFC的作用能够被哌唑嗪、阿替洛尔、ICI-118551、氨氯吡咪和哇巴因抑制。结论α肾上腺素能受体激动剂苯肾上腺素通过调节Na+-K+-ATP酶及钠通道的活性增加低氧肺损伤大鼠肺泡液体清除能力,促进肺水肿液的吸收。
[Abstract]:Objective to investigate the effect and mechanism of 伪 -adrenergic receptor agonist phenylephrine on alveolar fluid clearance rate (AFC) in hypoxic lung injury rats. Methods hypoxic rats were exposed to 10% oxygen concentration. Total lung water (TLW). Of AFC, was measured at 24 h and 48 h, respectively. Phenylephrine was mixed with 伪 1 receptor blocker prazosin, 伪 2 receptor blocker yohimbine, 尾 1 receptor blocker atenolol, 尾 2 receptor blocker ICI-118551, sodium channel blocker amclopimide and Na K ATPase blocker ouabain. In the alveolar cavity of rats exposed to hypoxia for 48 h, The changes of AFC were measured. Results AFC (17.09 卤2.36) / h after exposure to 10% oxygen concentration for 24 h was significantly lower than that in normal rats (16.85 卤2.87) / h. AFC (8.41 卤2.67) / r / h was significantly decreased at 48 h after exposure to 10% oxygen concentration. Phenylephrine can significantly increase AFC. in hypoxic rats The effects of phenylephrine on AFC in hypoxic rats were inhibited by prazosin, atenolol, ICI-118551, amlopimide and ouabain. Conclusion 伪 -adrenergic receptor agonist phenylephrine increases alveolar fluid clearance and pulmonary edema fluid absorption in hypoxic lung injury rats by regulating the activities of Na -K ATPase and sodium channels.
【作者单位】：中国医科大学附属盛京医院老年科;中国医科大学附属盛京医院;沈阳药科大学药物分析教研室;
【基金】：辽宁省科学技术计划项目(201202245) 教育部留学回国人员科研启动基金(教外司留1139)
【分类号】：R965.2

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