BDSF促进SD大鼠皮肤损伤和HEK-a细胞的修复能力

发布时间：2018-09-04 17:08

【摘要】：十二烷基癸烯酸(BDSF)是一种小分子短链脂肪酸,来源于细菌(Burkholderia cenocepacia),前人的研究发现其抑制白色念珠菌从酵母相向菌丝相的转变。本论文研究BDSF促进SD大鼠背部皮肤伤口修复,挖掘BDSF潜在药用价值,具体研究内容如下:1、综述皮肤损伤愈合的过程,损伤愈合过程近年来的研究进展以及本论文治疗SD大鼠皮肤损伤的药物十二烷基癸烯酸(BDSF)的来源和研究用途等。裸鼠体内构建肺癌肿瘤模型,概述不同构建方法的优缺点。2、研究BDSF对SD大鼠背部皮肤损伤的修复能力,SD大鼠制作皮肤损伤模型,药物治疗,ELISA法(酶联免疫吸附测定)测量血清中各细胞因子含量,苏木素伊红染色切片法和免疫组化切片等方法研究组织中各成分含量,从而研究BDSF对SD大鼠皮肤损伤修复能力的影响。研究发现,当BDSF为300μM时对皮肤的愈合修复效果最佳,伤口治疗第6天血管数目增多,第12天上皮化过程增强,表皮层增厚并向内延伸且内部组织排列紧密,细胞炎症因子(白介素1,白介素6等)含量下降,生长因子(血管内皮生长因子等)含量上升。研究表明BDSF能够促进背部皮肤伤口的修复。3、研究BDSF对由白色念珠菌(Candida albicans)造成HEK-a细胞损伤的修复能力,采用两两对比法和阳参归一法研究细胞因子芯片对44种细胞因子的测量。研究发现,当C.albicans组加入BDSF时,单核细胞趋化因子1(MCP-1)、重组人趋化因子(I-309)、粒细胞集落刺激因子(GCSF)、生长相关因子(GRO)、单核细胞趋化因子2(MCP-2)和肥大细胞生长因子(SCF)含量明显下降,表明BDSF有效促进由白色念珠菌造成的损伤修复。4、研究BDSF对裸鼠体内肿瘤模型及体外A549细胞的抑癌能力,采用建模、ELISA、切片等方法探究BDSF的效用,流式细胞仪和细胞PCNA试剂盒等方法研究BDSF对A549细胞增殖与凋亡的作用。研究发现,BDSF有微弱的抑制肿瘤细胞生长的作用,但作用强度不大。
[Abstract]:Dodecyldecenoic acid (BDSF) is a small molecular short chain fatty acid derived from the previous studies of bacterial (Burkholderia cenocepacia), which inhibited the transformation of Candida albicans from yeast to hyphae. In this paper, BDSF was used to promote the repair of back skin wounds in SD rats and explore the potential medicinal value of BDSF. The specific research contents are as follows: 1. The healing process of skin injury is summarized. The recent progress in the process of wound healing and the origin and application of dodecyldecenoic acid (BDSF) in the treatment of skin injury in SD rats were discussed in this paper. The tumor model of lung cancer was constructed in nude mice, and the advantages and disadvantages of different construction methods were summarized. 2. To study the repair ability of BDSF on back skin injury of SD rats and to establish skin injury model in SD rats. The contents of cytokines in serum were measured by Elisa, hematoxylin eosin staining and immunohistochemistry were used to study the contents of cytokines in tissues. To study the effect of BDSF on the repair ability of skin injury in SD rats. It was found that when BDSF was 300 渭 M, the healing effect of the skin was the best, the number of blood vessels increased on the 6th day, the epithelialization process increased on the 12th day, the epidermis thickened and extended inward, and the internal tissues were arranged closely. The content of cytokines (IL 1, IL 6, etc.) decreased, while the content of growth factors (Vascular Endothelial growth Factor, et al.) increased. The results showed that BDSF could promote the repair of back skin wounds. The ability of BDSF to repair HEK-a cells induced by Candida albicans (Candida albicans) was studied. The measurement of 44 cytokines by cytokine microarray was studied by pairwise contrast method and Yangshen normalization method. It was found that the contents of monocyte chemokine 1 (MCP-1), recombinant human chemokine (I-309), granulocyte colony stimulating factor (GCSF), growth related factor (GRO), monocyte chemokine 2 (MCP-2) and mast cell growth factor (SCF) (MGF) decreased significantly in C.albicans group. The results showed that BDSF could effectively promote the repair of damage caused by Candida albicans, study the inhibitory effect of BDSF on tumor models in nude mice and A549 cells in vitro, and explore the effect of BDSF by modeling ELISAand slicing. The effects of BDSF on proliferation and apoptosis of A549 cells were studied by flow cytometry and PCNA kit. It was found that BDSF had a weak inhibitory effect on tumor cell growth, but the effect was not significant.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R915

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