壳寡糖对HeLa细胞和A549细胞自噬性死亡的影响
发布时间：2018-09-07 09:02
【摘要】：为了探讨壳寡糖(COS)对He La细胞和A549细胞自噬性死亡的影响,利用不同浓度COS处理细胞后MTT法检测细胞的增殖抑制情况,采用MDC染色法观察自噬泡的形成,免疫荧光法检测LC3在细胞内的表达,Western-blot分析COS处理He La和A549细胞不同时间后细胞内LC3-Ⅱ/LC3-Ⅰ变化。结果表明,5.0 mg·m L-1COS对两种细胞的增殖抑制率最大,分别为52.15%和57.63%(P0.01)。与阴性对照相比,经COS处理后的细胞MDC染色细胞内荧光强度增加,且阳性信号聚集于核周区域;免疫荧光检测发现LC3阳性颗粒数明显增加,表明细胞内自噬体的数量增多。Western-blot检测分析随着COS处理细胞时间延长,LC3-Ⅱ/LC3-Ⅰ比值逐渐增大,显示细胞内自噬活性增强。由此推测COS能够通过诱导He La和A549细胞的自噬性死亡来抑制细胞增殖,为COS的抗肿瘤作用机制的研究提供理论依据。
[Abstract]:In order to investigate the effect of chitosan oligosaccharide (COS) on autophagy death of He La cells and A549 cells, the inhibition of cell proliferation was detected by MTT method after treated with different concentrations of COS, and the formation of autophagy was observed by MDC staining. The expression of LC3 in He La and A549 cells was detected by Western blot with immunofluorescence assay. The changes of LC3- 鈪，
本文编号：2227809
[Abstract]:In order to investigate the effect of chitosan oligosaccharide (COS) on autophagy death of He La cells and A549 cells, the inhibition of cell proliferation was detected by MTT method after treated with different concentrations of COS, and the formation of autophagy was observed by MDC staining. The expression of LC3 in He La and A549 cells was detected by Western blot with immunofluorescence assay. The changes of LC3- 鈪，
本文编号：2227809
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