维生素E对锰中毒小鼠海马区caspase-3表达的影响

发布时间：2018-09-11 19:17

【摘要】：目的:探讨维生素E对锰中毒小鼠学习记忆及海马区caspase-3表达的影响。方法:将60只雄性昆明小鼠随机分为四组:对照组(CG)、维生素E组(VG)、锰中毒组(MG)、锰中毒加维生素E组(MVG),每组15只。采用腹腔注射氯化锰的方式制作锰中毒模型,Morris水迷宫观察小鼠的学习记忆能力,免疫组织化学方法检测caspase-3蛋白的表达。结果:Morris水迷宫定位航行实验显示,CG、VG、MVG三组的逃避潜伏期比MG组明显缩短,空间探索实验中,CG、VG、MVG三组穿越平台的次数明显多于MG组。免疫组织化学实验中,MG组海马CA1区和DG区caspase-3的表达明显多于CG组,MVG组海马区caspase-3的阳性细胞数明显少于MG组。结论:维生素E干预可减少海马区神经细胞的凋亡,改善锰中毒小鼠的学习记忆能力。
[Abstract]:Objective: to investigate the effects of vitamin E on learning and memory and caspase-3 expression in hippocampus of manganese intoxicated mice. Methods: sixty male Kunming mice were randomly divided into four groups: control group, (CG), vitamin E group, (VG), manganese poisoning group, (MG), manganese poisoning group and vitamin E group (MVG), 15 rats in each group. The model of manganese poisoning was made by intraperitoneal injection of manganese chloride. Morris water maze was used to observe the learning and memory ability of mice. The expression of caspase-3 protein was detected by immunohistochemical method. Results the escape latency of the three groups was significantly shorter than that of the MG group, and the frequency of crossing the platform in the three groups was significantly higher than that in the MG group. The expression of caspase-3 in CA1 and DG was significantly higher in MVG group than in CG group. The number of caspase-3 positive cells in MVG group was significantly lower than that in MG group. Conclusion: vitamin E intervention can reduce apoptosis of hippocampal neurons and improve learning and memory ability of mice exposed to manganese.
【作者单位】：广西医科大学人体解剖学教研室;
【基金】：广西自然科学基金(2010GXNSFA013157)
【分类号】：R965

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