丁苯酞对慢性酒精中毒大鼠海马内硫化氢和纤维状肌动蛋白的影响

发布时间：2018-09-12 19:46

【摘要】：目的探究丁苯酞(NBP)对慢性酒精中毒大鼠学习记忆能力、海马内硫化氢(H2S)和纤维状肌动蛋白(F-actin)含量的影响。方法将50只SD雄性大鼠随机分为5组,除对照组外,将含酒的水作为大鼠唯一水来源,并逐渐增加酒精含量,采用自由饮用方法建立慢性酒精中毒模型。42 d后按慢性酒精中毒大鼠戒断评分表于末次饮酒后6 h检测大鼠模型,NBP低、中、高剂量组大鼠灌胃不同剂量NBP(大约14 d),饮酒结束后利用Y型电迷宫测试酒精中毒大鼠的学习记忆能力,随后检测海马组织H2S和F-actin的含量。结果与对照组大鼠学习记忆成绩(40.38±5.97)、H2S含量(23.25±3.61)和F-actin在阳性细胞中的表达相比,模型组大鼠学习记忆成绩(83.25±5.47)和H2S含量(43.86±6.95)均升高,F-actin的阳性表达降低(P0.01)。与模型组大鼠相比,NBP中、高剂量组大鼠学习记忆成绩(64.75±4.71)、(70.00±4.72)和H2S含量(32.21±5.32)、(36.78±7.29)nmol/g均降低,F-actin的阳性表达升高(P0.01)。结论 NBP能够减轻慢性酒精中毒大鼠对酒精的依赖,缓解酒精对大鼠学习记忆能力的影响,可能与NBP影响H2S和F-actin的含量有关。
[Abstract]:Objective to investigate the effects of butyphthalide (NBP) on learning and memory ability, content of hydrogen sulfide (H _ 2S) and fibrin actin (F-actin) in hippocampus of rats with chronic alcoholism. Methods 50 male SD rats were randomly divided into 5 groups. Except for the control group, the alcoholic water was used as the only source of water, and the alcohol content was increased gradually. The model of chronic alcoholism was established by free drinking method. After 42 days, the rats were measured for low and middle NBP according to the abstinence score table of rats with chronic alcoholism at 6 hours after the last drinking. Rats in high dose group were given different doses of NBP (about 14 d), after drinking). The learning and memory abilities of alcohol intoxicated rats were measured by Y-type electrical maze, and then the contents of H2S and F-actin in hippocampus were measured. Results compared with the learning and memory scores (40.38 卤5.97) and the expression of F-actin (23.25 卤3.61), the learning and memory scores (83.25 卤5.47) and the content of H2S (43.86 卤6.95) in the model group were significantly higher than those in the control group (P 0.01). Compared with the model group, the learning and memory scores (64.75 卤4.71), (70.00 卤4.72) and the content of H2S (32.21 卤5.32), (36.78 卤7.29) nmol/g in the high dose group decreased the positive expression of F-actin (P0.01). Conclusion NBP can reduce the dependence of alcohol on alcohol and alleviate the influence of alcohol on learning and memory ability of rats, which may be related to the effect of NBP on the contents of H 2S and F-actin.
【作者单位】：新乡医学院第二附属医院;新乡医学院;新乡医学院第三附属医院;河南省生物精神病学重点实验室;
【基金】：国家自然科学基金(81471351) 河南省教育厅课题(15A310006) 河南省卫生科技创新型人才工程专项(3052)
【分类号】：R965

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