无机砷诱导鼠类氧化损伤的Meta分析

发布时间：2018-09-12 21:26

【摘要】：目的系统评价砷(arsenic,As)的氧化损伤作用,为明确砷诱导的氧化损伤作用提供依据。方法 2名研究者独立地评价Cochrane、Pub Med、Web of Science、Springer和Medline等数据库的文献质量,提取资料并进行交叉核对。对纳入的研究结果采用Revman5.2和Stata12.0进行Meta分析。结果共纳入58篇文献。与正常对照组相比,砷能够明显降低机体SOD(SMD=-4.19)、GSH(SMD=-4.62)、GR(SMD=-4.66)、GST(SMD=-6.67)、GPx(SMD=-4.32)和CAT(SMD=-4.99)的水平,增加ROS(SMD=4.48)、MDA(SMD=4.68)和GSSG(SMD=5.39)的含量,差异均有统计学意义(P0.05);三价砷比五价砷更能降低GR的活性,提高MDA的含量(P0.05);相对于饮水染砷,灌胃方式更能降低CAT、GR、SOD、GST和GPx的活性,增加MDA、GSSG含量(P0.05);砷对组织中GSH水平的降低程度和ROS含量的增加程度均要要高于血液(P0.05);砷对出生不满60 d的实验鼠中GSH的减少量要高于60 d及以上者(P0.05);当砷干预剂量小于5 mg/kg(或5 mg/L)时,对GR的降低效果要明显高于5 mg/kg(或5 mg/L)及以上者;干预时间在30 d及以上时,砷对GPx活性的降低效果要低于30 d以下者(P0.05)。结论砷可以诱发显著的氧化损伤,降低抗氧化能力,损伤程度与砷的价态、剂量、处理方式和干预时间等因素有关。
[Abstract]:Objective to evaluate the oxidative damage of arsenic (arsenic,As) systematically and to provide evidence for determining the oxidative damage induced by arsenic. Methods two researchers independently evaluated the literature quality of Cochrane,Pub Med,Web of Science,Springer and Medline databases, extracted data and cross-checked them. Revman5.2 and Stata12.0 were used for Meta analysis. Results 58 articles were included. Compared with the normal control group, arsenic significantly decreased the levels of SOD (SMD=-4.19) SMD=-4.62 (SMD=-4.62) gr (SMD=-6.67) GST (SMD=-4.32) and CAT (SMD=-4.99), and increased the contents of ROS (SMD=4.48) MDA (SMD=4.68) and GSSG (SMD=5.39) (P0.05), trivalent arsenic significantly decreased the activity of GR and increased the content of MDA compared with pentavalent arsenic (P0.05). The activities of CAT,GR,SOD,GST and GPx were significantly decreased by gastric perfusion. Increase the content of MDA,GSSG (P0.05), decrease the level of GSH in tissues and increase the content of ROS in arsenic than in blood (P0.05); decrease the amount of GSH in mice with arsenic less than 60 days old than those with 60 days or more (P0.05); when the dose of arsenic intervention is higher than that of rats with less than 60 days of birth, the decrease of GSH is higher than that of mice with less than 60 days of age (P0.05). When it is less than 5 mg/kg (or 5 mg/L), The decreasing effect of arsenic on GPx activity was significantly higher than that on 5 mg/kg (or 5 mg/L) and more than 5 mg/kg, and when the intervention time was more than 30 days, the decrease effect of arsenic on GPx activity was lower than that of less than 30 days (P0.05). Conclusion arsenic can induce significant oxidative damage and decrease the antioxidant ability. The degree of arsenic damage is related to the valence, dose, treatment and intervention time of arsenic.
【作者单位】：石河子大学医学院;
【基金】：国家自然科学基金(81560517) 兵团重点领域科技攻关项目(2014BA039,2015AG014) 石河子大学国际合作项目(GJHZ201602)
【分类号】：R99

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