RP-HPLC法同时测定骨瓜提取物注射剂中15种氨基酸的含量
发布时间：2018-09-14 06:52
【摘要】：目的建立1种同时测定骨瓜提取物注射剂中15种氨基酸含量的RP-HPLC法。方法以异硫氰酸苯酯(PITC)为柱前衍生试剂对样品中15种氨基酸进行衍生化,采用Ocean C18(250 mm×4.6 mm,5μm)色谱柱,流动相A为水-乙腈(体积比1∶4),流动相B为浓度0.1 mol·L-1乙酸钠-乙腈(体积比97∶3),梯度洗脱,流速为1.0 m L·min-1,柱温为25℃,检测波长为254 nm,进样量为10μL。用内标法计算15种氨基酸的含量。结果 15种氨基酸质量浓度在1.24~160.72 mg·L-1内与峰面积呈良好的线性关系(r=0.997 7~1.000 0),15种氨基酸的平均加样回收率在97.7~102.4%内,加样回收率的RSD值在1.7%~3.3%内。结论该方法准确、可靠、灵敏,可以用于同时测定骨瓜提取物注射剂中15种氨基酸的含量。
[Abstract]:Objective to establish a RP-HPLC method for simultaneous determination of 15 amino acids in extract injection of Gugu melon. Methods 15 kinds of amino acids in the sample were derivatized with phenylethyl isothiocyanate (PITC) as precolumn derivatization reagent. The chromatographic column was Ocean C18 (250 mm 脳 4.6 mm,5 渭 m). Mobile phase A was water-acetonitrile (1:4 by volume ratio), mobile phase B was 0.1 mol L-1 sodium acetate acetonitrile (97:3), gradient elution, flow rate 1.0 mL min-1, column temperature 25 鈩，
本文编号：2241882
[Abstract]:Objective to establish a RP-HPLC method for simultaneous determination of 15 amino acids in extract injection of Gugu melon. Methods 15 kinds of amino acids in the sample were derivatized with phenylethyl isothiocyanate (PITC) as precolumn derivatization reagent. The chromatographic column was Ocean C18 (250 mm 脳 4.6 mm,5 渭 m). Mobile phase A was water-acetonitrile (1:4 by volume ratio), mobile phase B was 0.1 mol L-1 sodium acetate acetonitrile (97:3), gradient elution, flow rate 1.0 mL min-1, column temperature 25 鈩，
本文编号：2241882
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