沙利度胺对双膦酸盐相关颌骨坏死的影响

发布时间：2018-09-16 20:28

【摘要】：目的研究沙利度胺对双膦酸盐相关颌骨坏死的影响。方法 36只大鼠随机分成A、B、C组,分别接受生理盐水、唑来膦酸、唑来膦酸+沙利度胺治疗。用药3周后,拔除大鼠左上颌第1磨牙。拔牙后4、8周,收获标本,评价颌骨坏死、微血管生成及细胞凋亡情况。结果拔牙后4、8周,大鼠上颌骨拔牙创处均无死骨裸露,但B组、C组部分标本可见一些小的瘘管。组织学检查显示,A组标本未见死骨,而B组、C组在拔牙窝周围可见小块死骨。B组、C组拔牙窝周围区域空骨陷窝百分比和死骨面积显著多于A组(P0.01),而骨陷窝密度则显著下降。B组、C组微血管密度较A组也显著降低,在4周时分别下降了和25.87%和55.27%(P0.01),在8周时分别下降了45.62%和72.84%(P0.01);凋亡细胞数则在4周时分别增长了54.80%和87.89%(P0.01),在8周分别增长了208.08%和250.58%(P0.01)。结论沙利度胺加重了唑来膦酸诱发的早期阶段的颌骨坏死;沙利度胺和唑来膦酸对颌骨坏死的作用与微血管生成抑制有关。
[Abstract]:Objective to study the effect of thalidomide on bisphosphonate-associated osteonecrosis of jaws. Methods Thirty-six rats were randomly divided into two groups: group A, B, C, treated with normal saline and thalidomide zoledronate, respectively. After 3 weeks, the left maxillary first molar was extracted. Eight weeks after tooth extraction, specimens were harvested to evaluate jaw necrosis, microangiogenesis and apoptosis. Results No dead bone was exposed at the site of maxillary extraction in rats 4 weeks after tooth extraction, but some small fistula was found in some specimens of group B and C. Histological examination showed that no dead bone was found in group A. The percentage of empty bone lacunae and dead bone area in group C were significantly higher than those in group A (P0.01), while the density of bone lacunae in group C was significantly lower than that in group A (P0.01). The microvessel density of group C was significantly lower than that of group A (P 0.01). The number of apoptotic cells decreased by 54.80% and 87.89% (P0.01) at 4 weeks, and by 208.08% and 250.58% (P0.01) at 8 weeks, respectively, and decreased by 45.62% and 72.84% (P0.01) at week 4 and by 25.87% and 55.27% (P0.01) respectively at week 4, and by 45.62% and 72.84% (P0.01) at week 8, respectively, and the number of apoptotic cells increased by 54.80% and 87.89% (P0.01) respectively. Conclusion thalidomide exacerbates the early stage of jaw necrosis induced by zoledronic acid, and the effects of thalidomide and zoledronic acid on jaw necrosis are related to the inhibition of microangiogenesis.
【作者单位】：华北理工大学口腔医学院口腔颌面外科;华北理工大学基础医学院病理教研室;
【基金】：国家自然科学基金(81270965,81101448) 河北省自然科学基金(C2011401044)~~
【分类号】：R965

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