锌对胰岛素抵抗靶细胞活力和葡萄糖消耗量影响

发布时间：2018-10-08 13:51

【摘要】：目的探讨锌对胰岛素抵抗靶细胞活力和葡萄糖消耗量的影响。方法建成Hep G2细胞、3T3-L1细胞和L6成肌细胞胰岛素抵抗模型后,用10-7mol/L胰岛素和不同浓度锌(0、20、50、100、200、400μmol/L)孵育细胞,通过MTT法和葡萄糖氧化酶法测定基础状态和胰岛素刺激状态下3种胰岛素抵抗细胞葡萄糖的消耗量。结果不同剂量的锌孵育3种细胞3 h后,200、400μmol/L的锌可明显抑制各组细胞的活力(P0.05);50~100μmol/L的锌干预3 h能在不同程度上提高Hep G2、3T3-L1正常细胞和胰岛素抵抗细胞的葡萄糖消耗量,但其作用效果明显低于胰岛素;其中100μmol/L的锌与胰岛素共同作用的效果分别优于胰岛素或锌单独作用的效果(P0.05);对于L6肌细胞,20、50和100μmol/L锌均能明显提高基础状态下正常细胞和胰岛素抵抗细胞的葡萄糖消耗量(P0.05);仅有20μmol/L锌和胰岛素共同作用的效果优于胰岛素或锌单独作用的效果(P0.05)。结论不同浓度的锌可以在一定程度上提高3种胰岛素抵抗的靶细胞葡萄糖摄取量,且不同的细胞具有不同的适宜作用剂量。
[Abstract]:Objective to investigate the effect of zinc on the activity and glucose consumption of insulin resistant target cells. Methods the model of insulin resistance of Hep G2 cells was established. The cells were incubated with 10-7mol/L insulin and different concentrations of zinc (020 0100200400 渭 mol/L) after the establishment of insulin resistance model of 3T3-L1 cells and L6 myoblasts. Glucose consumption of three insulin resistant cells was measured by MTT method and glucose oxidase method. Results after three kinds of cells were incubated with different doses of zinc for 3 h, zinc of 200400 渭 mol/L could significantly inhibit the activity of each group (P0.05) the glucose consumption of Hep G2T3-L1 cells and insulin resistant cells could be increased in different degree after 3 h of zinc treatment with 50 渭 mol/L. But its effect was obviously lower than that of insulin. The interaction effect of zinc and insulin at 100 渭 mol/L was better than that of insulin or zinc alone (P0.05), and the glucose of normal cells and insulin resistant cells could be significantly increased in L6 muscle cells by 100 渭 mol/L and 20 渭 mol/L zinc (P0.05). Consumption (P0.05); only 20 渭 mol/L zinc and insulin combined effect was better than that of insulin or zinc alone (P0.05). Conclusion different concentrations of zinc can increase glucose intake of three insulin resistant target cells to a certain extent, and different cells have different appropriate dose.
【作者单位】：天津医科大学公共卫生学院;海洋石油总医院;
【基金】：国家自然科学基金(81172665) 天津市自然科学基金(10JCYBJC11100)
【分类号】：R96

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