罗格列酮、胰岛素对Ⅱ型糖尿病大鼠脑内TIPE2的表达和细胞凋亡的影响
发布时间：2018-10-10 15:00
【摘要】：目的探讨罗格列酮、胰岛素干预下Ⅱ型糖尿病大鼠脑内肿瘤坏死因子α诱导蛋白8样因子2(TIPE2)表达与细胞凋亡的变化,以及它们之间的相关性。方法选取3月龄Wistar大鼠(n=5,3Wistar组)和3月龄GK大鼠(n=5,3GK组);4月龄GK大鼠喂养至6月龄,根据期间的不同处理分为6月龄GK大鼠(n=5,6GK组)、罗格列酮干预6月龄GK大鼠(n=5,RSG+6GK组)、胰岛素干预6月龄GK大鼠(n=5,INS+6GK组)。采用免疫组化法检测各组大鼠脑内TIPE2的表达部位及特征,运用RT-PCR法和Western blotting法检测各组大鼠脑内TIPE2 mRNA和蛋白的表达,采用免疫荧光法检测各组大鼠脑内细胞凋亡的情况。结果 3GK组在染色强度、阳染细胞数、阳染血管数、mRNA、蛋白水平均较3 Wistar组有明显增加(P0.05),6GK组较3GK组有明显增加(P0.05),RSG+6GK组、INS+6GK组均较6GK组有明显减少(P0.05)。大鼠脑内细胞凋亡数目与TIPE2的表达呈正相关性(r=0.981 6,P0.05)。结论Ⅱ型糖尿病可促进大鼠脑内TIPE2的表达和细胞凋亡,随年龄的增长和糖尿病病程的进展,TIPE2的表达和细胞凋亡数目相应增加,罗格列酮、胰岛素干预可减少糖尿病大鼠脑内TIPE2的表达并抑制细胞凋亡。在糖尿病大鼠脑内细胞凋亡的进程中,TIPE2能够发挥促凋亡作用。
[Abstract]:Objective to investigate the relationship between the expression of tumor necrosis factor 伪 -inducible protein 8-like factor 2 (TIPE2) and apoptosis in the brain of diabetic rats induced by rosiglitazone and insulin. Methods 3-month-old Wistar rats (n = 5) and 3-month old GK rats (n = 5 GK) were selected, 4 months old GK rats were fed to 6 months old, and 4 months old GK rats were fed to the age of 6 months. According to the different treatments, the rats were divided into 6 month old GK rats (naglitazone group), rosiglitazone group (6 months old GK rats), insulin intervention 6 month old GK rats (nti5ins 6GK group), rosiglitazone treated GK rats at 6 months old (n = 5 RSG 6GK group) and insulin intervention 6 months old GK rats (n = 5 ins 6GK group). Immunohistochemical method was used to detect the expression of TIPE2 in rat brain, RT-PCR and Western blotting methods were used to detect the expression of TIPE2 mRNA and protein in rat brain, and apoptosis was detected by immunofluorescence method. Results the staining intensity, the number of positive staining cells, the number of positive stained blood vessels and the level of mRNA, protein in 3GK group were significantly higher than those in 3 Wistar group (P0.05), and the 6GK group was significantly higher than 3GK group (P0.05), RSG 6GK group, INS 6GK group was significantly lower than 6GK group (P0.05). There was a positive correlation between the number of apoptosis and the expression of TIPE2 in rat brain (r = 0.981 6 P 0.05). Conclusion Type 鈪，
本文编号：2262218
[Abstract]:Objective to investigate the relationship between the expression of tumor necrosis factor 伪 -inducible protein 8-like factor 2 (TIPE2) and apoptosis in the brain of diabetic rats induced by rosiglitazone and insulin. Methods 3-month-old Wistar rats (n = 5) and 3-month old GK rats (n = 5 GK) were selected, 4 months old GK rats were fed to 6 months old, and 4 months old GK rats were fed to the age of 6 months. According to the different treatments, the rats were divided into 6 month old GK rats (naglitazone group), rosiglitazone group (6 months old GK rats), insulin intervention 6 month old GK rats (nti5ins 6GK group), rosiglitazone treated GK rats at 6 months old (n = 5 RSG 6GK group) and insulin intervention 6 months old GK rats (n = 5 ins 6GK group). Immunohistochemical method was used to detect the expression of TIPE2 in rat brain, RT-PCR and Western blotting methods were used to detect the expression of TIPE2 mRNA and protein in rat brain, and apoptosis was detected by immunofluorescence method. Results the staining intensity, the number of positive staining cells, the number of positive stained blood vessels and the level of mRNA, protein in 3GK group were significantly higher than those in 3 Wistar group (P0.05), and the 6GK group was significantly higher than 3GK group (P0.05), RSG 6GK group, INS 6GK group was significantly lower than 6GK group (P0.05). There was a positive correlation between the number of apoptosis and the expression of TIPE2 in rat brain (r = 0.981 6 P 0.05). Conclusion Type 鈪，
本文编号：2262218
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