硫柳汞对秀丽隐杆线虫神经行为及生长发育的影响

发布时间：2018-10-12 12:44

【摘要】：目的通过研究硫柳汞对秀丽隐杆线虫(简称线虫)幼虫神经行为及生长发育的影响,初步探讨硫柳汞对线虫的神经发育毒性。方法采用L1期线虫,以0~140μmol/L或0~5μmol/L硫柳汞分别暴露48 h,检测其半数致死浓度(LC50),分别考察其对线虫运动行为、感知行为、摄食行为及生长发育(体长、体宽)的影响。结果与溶剂对照组比较,线虫幼虫暴露于硫柳汞48 h后,其运动行为(头部摆动和身体弯曲)、感知行为(趋向和趋避)和摄食行为(咽泵震动)均有显著改变(P0.05)。此外,硫柳汞显著抑制线虫的生长发育,并且这种抑制作用具有剂量-效应关系。结论硫柳汞对线虫幼虫神经行为及生长发育产生不良影响,其毒性机制有待深入研究。
[Abstract]:Objective to investigate the neurodevelopmental toxicity of thiomersal to nematode by studying the effect of thiomersal on the nerve behavior and growth of nematode larvae. Methods the 50% lethal concentration (LC50) of nematode was measured after exposure to 0 ~ 140 渭 mol/L or 0 ~ 5 渭 mol/L thiomersal for 48 h, and the effects on motor behavior, perceptual behavior, feeding behavior and growth and development (body length, body width) of nematode were investigated. Results compared with the solvent control group, the movement behavior (head swing and body bending), perceptual behavior (trend and avoidance) and feeding behavior (pharyngeal pump vibration) of nematode larvae significantly changed after exposure to thiomersal for 48 h (P0.05). In addition, thiomersal significantly inhibited the growth and development of nematode, and the inhibition was dose-effect. Conclusion thiomersal has adverse effects on nervous behavior, growth and development of nematode larvae, and the toxic mechanism of thiomersal needs further study.
【作者单位】：北京大学公共卫生学院毒理学系;北京市疾病预防控制中心/北京市预防医学研究中心/食物中毒诊断溯源技术北京市重点实验室;首都医科大学公共卫生学院;食品安全毒理学研究与评价北京市重点实验室;国家中医药管理局中药配伍减毒重点研究室;
【基金】：国家自然科学基金(81673685,81273108) 首都卫生发展科研专项(首发2011-1013-03)
【分类号】：R965

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