维甲酸对皮肤角阮细胞水孔蛋白通道3表达的影响

发布时间：2018-10-20 11:47

【摘要】：目的探讨维甲酸类药物对表皮角阮细胞中水孔蛋白通道(AQP)3表达的影响。方法异维A酸、阿维A、阿达帕林处理角朊细胞的体外培养模型(Ha Ca T),采用免疫组化、RT-PCR、Western印迹分子生物学方法检测不同浓度(0.000、0.001、0.010、0.060、0.100 mg/ml)、不同作用时间(0、6、12、24、48 h)及不同干预因素下对Ha Ca T中AQP3含量的变化。结果 1浓度为0.010 mg/ml时,三组Ha Ca T中AQP3的表达均高于其他浓度(P0.05);20.010 mg/ml浓度的异维A酸、阿维A、阿达帕林作用12 h,三组Ha Ca T中AQP3的表达异维A酸组阿维A组阿达帕林组,组间比较差异显著(P0.05)。结论维甲酸可调节Ha Ca T中AQP3的表达,0.010 mg/ml浓度的异维A酸组12 h的时候对Ha Ca T中AQP3表达的上调作用最明显。
[Abstract]:Objective to investigate the effect of retinoic acid on the expression of aquaporin channel (AQP) 3 in keratokeratoin cells. Methods Isoretinoic acid, (Ha Ca T), an in vitro culture model of keratinocytes treated with Avera and Adalparine, was used to detect the changes of AQP3 content in Ha Ca T at different concentrations (0. 000,0. 001P0. 01010, 0. 060,0. 100 mg/ml) and different time (0. 060? 2448h) and different intervention factors by immunohistochemistry and RT-PCR,Western blot molecular biology. Results (1) at 0.010 mg/ml, the expression of AQP3 in Ha Ca T of the three groups was higher than that of the other groups (P0.05), the expression of AQP3 in Ha Ca T of the three groups was increased after 12 h exposure to isoretinoic acid, avea and adalapalin at the concentration of 20.010 mg/ml, and the expression of AQP3 in the Ha Ca T of the three groups was higher than that of the control group (P0.05). There was significant difference between the two groups (P0.05). Conclusion Retinoic acid can regulate the expression of AQP3 in Ha Ca T, and the up-regulation of AQP3 expression in Ha Ca T is most obvious in 0.010 mg/ml isoretinoic acid group at 12 h.
【作者单位】：江西中医药高等专科学校人体解剖学教研室;
【分类号】：R96

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