蒿甲醚联合依托泊苷对小细胞肺癌细胞株H446增殖及侵袭的抑制作用

发布时间：2018-10-26 16:01

【摘要】：探讨蒿甲醚与依托泊苷联用对小细胞肺癌细胞H446增殖及侵袭能力的抑制作用。采用MTT法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞增殖的影响;采用Annexin V/PI荧光双染法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞凋亡的影响;采用PI单染法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞周期分布的影响。采用基质胶侵袭实验检测蒿甲醚、依托泊苷单用及联合作用对H446侵袭能力的影响。结果表明,蒿甲醚、依托泊苷单用以及联合使用均能有效抑制H446的增殖,且呈剂量依赖性,两药联用具有协同作用。蒿甲醚单用不能明显促进细胞凋亡,但与依托泊苷(300 nmol/L)联用72 h可以比较明显促进H446凋亡,具有统计学意义(P0.05)。蒿甲醚单用对H446的细胞周期没有显著影响,联合作用48 h对细胞周期G_2期阻滞主要是依托泊苷的作用。单用蒿甲醚、依托泊苷以及二者联合使用都能显著抑制H446的侵袭能力。
[Abstract]:To investigate the inhibitory effect of artemether and etoposide on the proliferation and invasion of small cell lung cancer cell line H446. The effects of artemether, etoposide alone and combined action on the proliferation of H446 cells were detected by MTT assay, and the effects of artemether and etoposide alone and combined action on the apoptosis of H446 cells were detected by Annexin V/PI fluorescence double staining. The effects of artemether, etoposide alone and combined action on cell cycle distribution of H446 cells were detected by PI single staining. The effects of artemether, etoposide alone and combined action on the invasion ability of H 446 were detected by matrix gel invasion test. The results showed that artemether etoposide alone and combined use could effectively inhibit the proliferation of H446 in a dose-dependent manner. Artemether alone could not significantly promote apoptosis, but combined with etoposide (300 nmol/L) for 72 h could significantly promote apoptosis of H446 (P0.05). Artemether alone had no significant effect on the cell cycle of H446. The combined action of 48 h on cell cycle arrest was mainly caused by etoposide. Artemether alone, etoposide and their combination could significantly inhibit the invasion ability of H 446.
【作者单位】：南京大学生命科学院;
【基金】：国家自然科学基金资助项目(No.81473220)~~
【分类号】：R96

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