不同血浆代用品对大鼠血糖和电解质影响的研究

发布时间：2018-10-29 12:22

【摘要】：目的比较不同血浆代用品对大鼠血糖和电解质的影响。方法雄性Wistar大鼠随机分为5组(10只/组),使用琥珀酰明胶(GEL)、羟乙基淀粉200(HES200)、羟乙基淀粉130(HES130)、羟乙基淀粉40(706)和右旋糖酐40(Dex40)分别行重度急性等容血液稀释(ANH),剂量为94.76 m L/kg,用血气分析仪检测基础值、血液稀释结束和血液稀释后1 h的血糖和电解质浓度。结果动脉与静脉的血糖、钾离子和钙离子浓度(mmol/L)分别为7.50±0.50 vs6.87±0.57(P0.01)、3.72±0.18 vs3.68±0.21(P0.05)、1.368±0.039 vs1.289±0.08(P0.01);动脉与静脉的钠离子和氯离子浓度(mmol/L)分别为142.0±1.4 vs 143.4±1.7(P0.01)、105.06±2.26 vs106.08±2.96(P0.01)。GEL和Dex40组在血液稀释结束后血糖浓度明显上升(P0.05或0.01),HES130和706组在血液稀释1 h血糖浓度明显上升(P0.05)。Dex40组在血液稀释1 h钾离子浓度明显高于其他组(P0.01)。GEL、HES200、HES130和Dex40组在血液稀释1h后钙离子浓度明显上升(P0.05或0.01)。血液稀释引起各组氯离子浓度明显上升(P0.01),同时GEL组的氯离子浓度明显低于其他组(P0.01)。结论血浆代用品制备ANH的大鼠模型,其血糖和电解质发生紊乱,血液稀释后Dex40引起血钾异常升高。
[Abstract]:Objective to compare the effects of different plasma substitutes on blood glucose and electrolyte in rats. Methods male Wistar rats were randomly divided into 5 groups (10 rats per group). Succinyl gelatin (GEL), hydroxyethyl starch 200 (HES200), hydroxyethyl starch 130 (HES130) were used. Hydroxyethyl starch 40 (706) and dextran 40 (Dex40) were treated with severe acute isovolemic hemodilution (ANH),) at a dose of 94.76 mL / kg, respectively. Blood glucose and electrolyte concentration 1 hour after hemodilution and hemodilution. Results the concentrations of blood glucose, potassium ion and calcium ion (mmol/L) in artery and vein were 7.50 卤0.50 vs6.87 卤0.57, 3.72 卤0.18 vs3.68 卤0.21 (P0.05), 1.368 卤0.039 vs1.289 卤0.08 (P0.01), respectively. The concentrations of sodium ion and chloride ion (mmol/L) in artery and vein were 142.0 卤1.4 vs 143.4 卤1.7 (P0.01), respectively. 105.06 卤2.26 vs106.08 卤2.96 (P0.01). GEL and Dex40) significantly increased blood glucose concentration after hemodilution (P0.05 or 0.01). The concentration of blood glucose in HES130 and 706 groups increased significantly at 1 h of hemodilution (P0.05). The concentration of potassium ion in Dex40 group was significantly higher than that in other groups (P0.01). GEL,HES200, in Dex40 group was significantly higher than that in other groups (P0.01). After 1 hour of hemodilution, calcium concentration in HES130 and Dex40 groups increased significantly (P0.05 or 0.01). The concentration of chloride in GEL group was significantly lower than that in other groups (P0.01). Conclusion the rat model of ANH was induced by plasma substitute, the blood glucose and electrolyte were disturbed, and Dex40 caused abnormal increase of serum potassium after hemodilution.
【作者单位】：军事医学科学院野战输血研究所;
【基金】：国家高技术研究发展计划(“863”)(2012AA021902) 国家自然科学基金面上项目(31271001)
【分类号】：R965

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