羰基镍急性中毒大鼠血清镍变化与淋巴细胞DNA损伤及加合物形成的研究

发布时间：2018-10-29 13:45

【摘要】：目的观察羰基镍急性中毒大鼠血清镍变化与淋巴细胞DNA损伤及加合物形成情况,探讨羰基镍急性中毒致大鼠淋巴细胞DNA损伤的机制。方法染毒后第1、2、3和7天,测定4个染毒组(A、B、C组分别为低、中、高浓度羰基镍染毒组,D组为氯气染毒组)与正常对照组(E组)大鼠的血清镍。采用紫外吸收光谱移动法测定淋巴细胞DNA加合物,单细胞凝胶电泳观察DNA损伤。结果各染毒组血清镍在染毒后第1天最高,随时间的推移逐渐降低。各染毒组淋巴细胞DNA均在259 nm处有最高吸收峰,与E组(在260 nm处有最高吸收峰)比较位移均为+1 nm。A组淋巴细胞DNA最大吸光度在第3天降至最低点,B组在第7天降至最低点,C组和D组在第2天降至最低点,与E组比较,差异均有统计学意义(P0.05)。各染毒组淋巴细胞DNA彗星尾长和Olive尾矩均大于正常对照组(P0.05)。结论羰基镍急性染毒后第1天大鼠血清镍最高。染毒后第2天淋巴细胞DNA加合物形成最多,第3天淋巴细胞DNA损伤最重,第7天有所恢复。
[Abstract]:Objective to observe the changes of serum nickel and lymphocyte DNA damage and the formation of adducts in rats with acute carbonyl nickel poisoning, and to explore the mechanism of lymphocyte DNA damage induced by acute carbonyl nickel poisoning in rats. Methods on the 3rd and 7th day after exposure, serum nickel was measured in 4 groups (low, middle and high concentrations of carbonyl nickel and chlorine in group D) and normal control group (group E). The DNA adducts of lymphocytes were determined by UV absorption spectrometry and DNA damage was observed by single cell gel electrophoresis. Results Serum nickel was highest on the first day after exposure, and decreased gradually with time. The highest absorption peak of lymphocyte DNA was at 259 nm in each exposed group, and the shift of the maximum absorbance of lymphocyte DNA in 1 nm.A group was lower than that in E group (260 nm) on the 3rd day, and the maximum absorbance of Lymphocyte DNA decreased to the lowest point on the 3rd day, compared with that of E group (260 nm). Group B reached the lowest point on the 7th day, group C and group D reached the lowest point on the second day, compared with group E, the difference was statistically significant (P0.05). The tail length of DNA comet and the tail moment of Olive in lymphocytes of each exposed group were higher than those of normal control group (P0.05). Conclusion the serum nickel concentration was the highest in rats on day 1 after acute exposure to carbonyl nickel. The lymphocyte DNA adducts were most formed on the second day after exposure, and the DNA damage on the third day was the most serious, and recovered on the 7th day.
【作者单位】：兰州大学公共卫生学院流行病与卫生统计学研究所;兰州大学甘肃省新药临床前研究重点实验室;金川集团股份有限公司职工医院;
【基金】：金川集团公司与兰州大学产学研合作项目(金科综2009-12和2011-18)
【分类号】：R99

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