A型肉毒毒素对P物质引发的离体食管下括约肌收缩的抑制作用

发布时间：2018-11-03 11:04

【摘要】：目的观察A型肉毒毒素(BTX-A)对电场刺激(EFS)、内源性及外源性P物质(SP)引发的SD大鼠离体食管下括约肌(LES)收缩的影响。方法 SD大鼠叩击头部致昏后剪取食管下段与贲门交界处组织制备肌条。使用EFS诱发肌条内源性递质释放,阿托品阻断胆碱受体作用后,观察NK1受体拮抗剂(APTL-SP)、BTX-A对LES收缩的影响;观察不同浓度APTL-SP、BTX-A,对外源性SP引发的LES收缩变化影响。采用Biolap BL-420E生物机能实验系统同步记录肌条的收缩变化曲线。结果 EFS可增强LES的收缩张力及振幅(P0.01),BTX-A、阿托品、APTL-SP均可单独抑制其效应(P0.05或P0.01),而阿托品作用后BTX-A可进一步降低LES的收缩张力及振幅(P0.05);EFS的增强LES收缩效应,在被阿托品抑制后,可被后加入的APTL-SP进一步抑制(P0.05);阿托品与APTL-SP对EFS引发的LES收缩的协同抑制效应和BTX-A的抑制效应对比,二者无统计学差异(P0.05)。外源性加入的SP可显著增强LES收缩张力(P0.01)及振幅(P0.05)。结论 BTX-A可抑制EFS诱发的内源性SP引发的LES缩增强,其对外源性SP引发的LES收缩也具有抑制效应。
[Abstract]:Objective to observe the effect of botulinum toxin A (BTX-A) on the contraction of (LES) in isolated esophageal sphincter of SD rats induced by endogenous and exogenous substance P (SP) in (EFS), stimulated by electric field. Methods SD rats were knocked out to make muscle strips at the junction of lower esophagus and cardia. The effects of NK1 receptor antagonist (APTL-SP) and BTX-A on the contraction of LES were observed after the release of endogenous transmitters from muscle strips induced by EFS and the blocking of choline receptor by atropine. The effects of different concentrations of APTL-SP,BTX-A, on LES contraction induced by exogenous SP were observed. The contraction curve of muscle strips was recorded synchronously by Biolap BL-420E biological function experiment system. Results EFS could enhance the contractile tension and amplitude of LES (P0.01). BTX-A, atropine and APTL-SP could inhibit the contractile tension and amplitude of LES alone (P0.05 or P0.01). The contractile tension and amplitude of LES were further decreased by BTX-A after atropine treatment (P0.05). The contractile effect of LES enhanced by EFS was inhibited by atropine and further inhibited by APTL-SP added after Atropine (P0.05). There was no significant difference between atropine and APTL-SP in the synergistic inhibition of LES contraction induced by EFS and the inhibitory effect of BTX-A (P0.05). Exogenous SP significantly increased the contractile tension and amplitude of LES (P0.05). Conclusion BTX-A can inhibit LES contraction induced by endogenous SP induced by EFS and LES contraction induced by exogenous SP.
【作者单位】：漯河医学高等专科学校基础医学部;郑州大学基础医学院解剖学教研室;
【基金】：河南省教育厅自然科学研究资助项目(2011C310012) 漯河医学高等专科学校自然科学研究资助项目(2010S11)
【分类号】：R965

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