载IR-780与多柔比星温敏脂质体的制备与表征

发布时间：2018-11-16 11:02

【摘要】：目的制备同时包封IR-780和多柔比星(DOX)的温敏脂质体并进行表征。方法采用薄膜水化法及硫酸铵梯度法制备载IR-780和DOX温敏脂质体(DOX-IR-780thermo-sensitive liposome,DITSL)。采用Malvern激光粒度仪检测各脂质体的粒径、表面电位及多分散系数(PDI);采用激光诱导DITSL升温释药,检测各脂质体的释药特性。结果 IR-780和DOX同时被包封于温敏脂质体,成功制备得DITSL。IR-780和DOX的包封率分别为(94.47±8.57)%、(92.52±7.61)%;平均粒径(138.98±8.74)nm;略带负电位;PDI为0.32±0.02。激光0.8 W/cm2照射5min,温度最高升到54.2℃,DOX释药率达80.1%。结论 DITSL具有药物包封率较高、粒径大小适宜、光热转化效率高、温度敏感性好的优点,并可通过激光控制释药,为下一步光热-化疗联合治疗肿瘤的研究奠定了基础。
[Abstract]:Objective to prepare and characterize the thermo-sensitive liposomes which encapsulate both IR-780 and doxorubicin (DOX) simultaneously. Methods IR-780 and DOX thermo-sensitive liposomes (DOX-IR-780thermo-sensitive liposome,DITSL) were prepared by membrane hydration and ammonium sulfate gradient method. The particle size, surface potential and polydispersity coefficient of liposomes were measured by Malvern laser particle size analyzer. The release characteristics of liposomes were detected by laser induced DITSL. Results the encapsulation efficiency of DITSL.IR-780 and DOX were (94.47 卤8.57)% and (92.52 卤7.61)%, respectively, and the mean diameter of DITSL.IR-780 and DOX were (138.98 卤8.74) nm; with negative potential. PDI was 0.32 卤0.02. When the laser was irradiated with 0.8 W/cm2 for 5 minutes, the highest temperature was 54.2 鈩，

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