纳米银对人冠状动脉内皮细胞凋亡的影响及其机制

发布时间：2018-11-16 12:01

【摘要】：目的探讨纳米银对人冠状动脉内皮细胞(HCAEC)凋亡的影响及其可能的机制。方法取生长状态良好并处于对数生长期的HCAEC,分别加入浓度为0、2.5、5、10、20、40、80μg/m L的纳米银溶液,作用24 h。采用MTT法检测HCAEC凋亡率,ELISA法检测培养液上清血红素氧合酶1(HO-1)、血小板内皮细胞黏附分子1(PECAM-1)表达,火焰原子吸收光谱法检测细胞内、外银含量。结果加入2.5、5、10、20、40、80μg/m L纳米银溶液的HCAEC凋亡率分别为8.51%、21.03%、42.38%、64.62%、78.35%、74.83%。随着加入纳米银溶液浓度升高,HCAEC培养液上清HO-1表达逐渐升高,各浓度间比较P均0.05;加入40、80μg/m L纳米银溶液的HCAEC培养液上清HO-1表达比较P0.05。HCAEC加入10、20、40、80μg/m L纳米银溶液后细胞培养液上清PECAM-1表达均高于0、2.5、5μg/m L纳米银溶液,且加入40、80μg/m L纳米银溶液后PECAM-1表达升高更明显(P均0.05)。加入2.5、5、10、20μg/m L纳米银溶液的HCAEC细胞内银含量低于细胞外(P均0.01),加入40、80μg/m L纳米银溶液的HCAEC细胞内、外银含量比较差异均无统计学意义(P均0.05)。结论纳米银可促进HCAEC凋亡,其细胞毒性作用呈浓度依赖性;通过上调HO-1、PECAM-1表达造成细胞损伤可能是其作用机制。
[Abstract]:Objective to investigate the effect of silver nanoparticles on apoptosis of human coronary artery endothelial cells (HCAEC) and its possible mechanism. Methods HCAEC, which grew well and was in logarithmic growth stage, was treated with silver nanocrystalline solution of 4080 渭 g / mL at the concentration of 0 ~ 2.5g / m ~ (-1) 10 ~ (10) C ~ (-1) for 24 h. Apoptosis rate of HCAEC was detected by MTT assay, heme oxygenase 1 (HO-1) was detected by ELISA method, adhesion molecule 1 (PECAM-1) expression of platelet endothelial cells was detected by ELISA method, and silver content inside and outside cells was detected by flame atomic absorption spectrometry. Results the apoptotic rate of HCAEC was 8.51% and 42.38%, respectively. The apoptosis rate of HCAEC was 78.35% and 74.83%, respectively. With the increase of the concentration of silver nanoparticles, the expression of HO-1 in the supernatant of HCAEC medium increased gradually, P 0.05; Comparison of HO-1 expression in the supernatant of HCAEC cultured with 40 ~ 80 渭 g / mL silver nanocrystalline solution; the expression of PECAM-1 in the supernatant of the cell culture medium was higher than that in 0.5 渭 g / mL silver nanoparticles solution after the addition of 10 ~ 20 渭 g / mL silver nanocrystalline solution to 10 ~ 20 渭 g / m ~ (-1) silver nanocrystalline solution. In addition, the expression of PECAM-1 increased significantly after adding 40 渭 g / mL silver nanoparticles (P0. 05, P < 0. 05). The silver content of HCAEC cells added with 2.5 渭 g / mL silver nanoparticles was lower than that in HCAEC cells (P0.01), but there was no significant difference in silver content in HCAEC cells with 4080 渭 g / mL silver nanoparticles (P0. 05). Conclusion Silver nanoparticles can promote the apoptosis of HCAEC in a concentration dependent manner, and its mechanism may be the damage caused by up-regulation of HO-1,PECAM-1 expression.
【作者单位】：华北石油管理局总医院;
【分类号】：R99

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