高效液相色谱法测定普依司他盐酸盐原料药的含量
发布时间：2018-11-24 20:17
【摘要】：目的:建立高效液相色谱法测定普依司他盐酸盐原料药含量。方法:采用Waters Xbridge C18色谱柱(150 mm×4.6 mm,5μm),以0.01 mol·L~(-1)磷酸二氢钠缓冲液(p H=3.0)-乙腈为流动相,梯度洗脱,流速1.0 m L·min~(-1),柱温30℃,检测波长280 nm。结果:普依司他盐酸盐在6.25~200μg·m L~(-1)范围内,峰面积与浓度呈良好线性关系(R2=0.999 7,n=6)。平均回收率为100.40%(RSD=1.13%,n=9)。结论:该方法简便快速、准确灵敏、重复性好,可用于普依司他盐酸盐原料药含量测定。
[Abstract]:Objective: to establish a HPLC method for the determination of priestat hydrochloride. Methods: Waters Xbridge C18 column (150 mm 脳 4.6 mm,5 渭 m),) was used as mobile phase with 0.01 mol L ~ (-1) sodium dihydrogen phosphate buffer solution (pH 3.0-acetonitrile) as mobile phase, gradient elution, flow rate 1.0 mL min~ (-1). Column temperature 30 鈩，
本文编号：2354908
[Abstract]:Objective: to establish a HPLC method for the determination of priestat hydrochloride. Methods: Waters Xbridge C18 column (150 mm 脳 4.6 mm,5 渭 m),) was used as mobile phase with 0.01 mol L ~ (-1) sodium dihydrogen phosphate buffer solution (pH 3.0-acetonitrile) as mobile phase, gradient elution, flow rate 1.0 mL min~ (-1). Column temperature 30 鈩，
本文编号：2354908
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