两亲性嵌段共聚物普朗尼克的细胞毒性及细胞摄取

发布时间：2018-12-09 20:57

【摘要】：目的:考察不同型号、混合普朗尼克对MCF-7及耐药MCF-7的细胞毒性和细胞摄取,筛选出可供后续胶束研究的混合普朗尼克最优配比。方法:选取Pluronic F68、F127、P85、P105、P123,按HLB值分为2组,以一定比例交叉混合,采用CCK-8法和高效液相色谱法,测定普朗尼克、混合普朗尼克对MCF-7及耐药MCF-7的细胞毒性和细胞摄取量。结果:试验结果表明,F68、F127对细胞的毒性较小,而P85、P105、P123对细胞的毒性相对较大;当2种普朗尼克配比使用的质量浓度小于100μg·mL-1时,MCF-7及耐药MCF-7 2种细胞的存活率均大于70%。随着混合普朗尼克的浓度增加,细胞毒性均逐渐增强;F68与P85配比使用时,细胞毒性最大,且配比浓度为1∶4时的细胞毒性大于1∶2。Pluronic P85、P105、P123均可促进肿瘤细胞的药物摄取,Pluronic F68、F127基本不促进药物的摄取。F127-P123较其他混合普朗尼克表现出更强的摄取能力。结论:随着HLB值的降低及浓度的增大,普朗尼克对MCF-7及耐药MCF-7细胞的毒性增加。混合普朗尼克可以促进肿瘤细胞对于化疗药物的摄取。
[Abstract]:Aim: to investigate the cytotoxicity and cellular uptake of MCF-7 and drug-resistant MCF-7 by different types of mixed Plannick and to select the optimal mixture ratio for further micelle study. Methods: Pluronic F68, F127, P85, P105, P123, were divided into two groups according to the HLB value. The mixture was cross-mixed in a certain proportion. Plannick was determined by CCK-8 and HPLC. Cytotoxicity and uptake of mixed Plannick to MCF-7 and MCF-7. Results: the results showed that the toxicity of F68 F127 was less than that of P85 P105 P123. When the mass concentration of two kinds of Plannick was less than 100 渭 g mL-1, the survival rates of both MCF-7 and drug-resistant MCF-7 were higher than 70%. With the increase of the concentration of mixed Plannick, the cytotoxicity increased gradually. When F68 and P85 were used together, the cytotoxicity was the greatest, and the cytotoxicity at 1:4 was higher than that of 1:2.Pluronic P85, P105 and P123. Pluronic F68 could promote the drug uptake of tumor cells. F 127 does not promote drug uptake. F127-P123 has a stronger uptake capacity than other mixes of Plannick. Conclusion: with the decrease of HLB and the increase of concentration, the toxicity of Plannick to MCF-7 and MCF-7 cells is increased. Mixed Plannick can promote the uptake of chemotherapeutic drugs by tumor cells.
【作者单位】：石河子大学药学院;新疆军区总医院药剂科;
【基金】：国家自然科学基金(编号:81372782) 新疆自治区科技厅自然科学基金(编号:2015211C242)
【分类号】：R943

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